Immunohistochemical study on the changes of sugar chain in cervical malignancies.

宫颈恶性肿瘤糖链变化的免疫组化研究

基本信息

批准号：
60570784
负责人：
SUGIMORI Hajime
金额：
$ 1.22万
依托单位：
Saga Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

项目摘要

The cervical tissues including normal cervix, squamous metaplasia, dysplasia, carcinoma in situ and invasive carcinoma, were studied by lectin histochemistry. DBA, PNA, SBA, MPA, RCA-1 and GS-1 which combine to galactose or N-acety galactosamine, bound to superficial and partly intermediate layer of normal squamous epithelium and demonstrated the increasing affinity of these lectins in accordance with the epithelial maturation. This staining characteristics was lost in dysplasia and carcinoma indicating maturation abnormality of these lesions. These lectins were positive in center of the cancer pearl in well differentiated squamous cell carcinoma. This finding corresponds with DBA positive finding in pearl-like accumulated area of SKG III cell in culture. N- acety galactosamine binding lectin was negative in the normal cervical gland, byt was strongly positive in the cervical adenocarcinoma clearly indicating the change of sugar chain in accordance with malignant transformation. This positivity was not observed in endometrial adenocarcinoma, and it was considered that this staining could serve for differentiation of both adenocarcinoma. Because apparent change of sugar chain was observed in mucus producing cells, lectin staining was applied to mucinous adenocarcinoma of the ovary. Lectin was strongly positive in this type of carcinoma showing strong contrast with negative staining to serous adenocarcinoma of the ovary. In study of cultures cells DBA and PBA was strongly positive in cell in mitosis. This finding will introduce the change of sugar chain during cell cycle, which requests further research.

通过凝集素组织化学研究了宫颈组织，包括正常子宫颈，鳞状化生，发育不良，原位癌和侵入性癌。 DBA，PNA，SBA，MPA，RCA-1和GS-1结合了半乳糖或N型半乳糖胺，与正常鳞状上皮的表面和部分中间层结合，并证明了这些位位属蛋白的亲和力的增加，根据上皮成熟。这种染色特征在发育不良和癌中丧失，表明这些病变的成熟异常。这些凝集素在分化良好的鳞状细胞癌中心的癌症中心呈阳性。这一发现与培养物中SKG III细胞的珍珠样积累区域的DBA阳性发现相对应。正常宫颈腺体的N-乙酰乳糖酰胺结合凝集素为阴性，在宫颈腺癌中，ByT明显地表明，根据恶性转化，糖链的变化。在子宫内膜腺癌中未观察到这种阳性，并且认为这种染色可以用于分化两种腺癌。由于在产生粘液的细胞中观察到糖链的明显变化，因此将凝集素染色应用于卵巢的粘液腺癌。在这种类型的癌中，凝集素与卵巢浆液性腺癌的阴性染色相对鲜明鲜明对比。在研究培养物细胞和PBA中，有丝分裂的细胞呈阳性。这一发现将在细胞周期中引入糖链的变化，这需要进一步研究。