Effect of hormones on carcinogenesis of cervical cancer.

激素对宫颈癌癌变的影响。

• 批准号: 04671010
• 负责人: SUGIMORI Hajime
• 金额: $ 1.34万
• 依托单位: Saga Medial School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671010/
• 关键词: Cervical cancer; Oncogene; Hormone; 子宮頚癌; 性ホルモン; 増殖因子; 癌抑制遺伝子; 上皮増殖因子

The importance of infection of human papilloma virus (HPV) was clarified by clinical and in vitro investigations. Not only in squamous cell carcinoma but also in adenocarcinoma HPV was noted in high frequency. Abnormal suppressor oncogene p53 was observed in HPV negative cancer cell lines, while only wildtype p53 was detected in HPV positive cell lines. A new immortalized cell line was established by introducing HPV 18 to the secondly culture cells from the normal cervix. This cell lines derived from endocervical cells showed strong atypia and cytokeratin resembled to HeLa cells which was adenocarcinoma cell line. This cell line will be useful for studying the carcinogenesis of adenocarcinoma of the cervix. The patients with dysplasia were followed up. High risk HPV (type 16/18) was frequently detected in patients with age of 30-40s. Progressed cases were mainly observed in 40s. There were few cases of progression or regression in patients over 50 year-old. This fact clearly indicated that progres or regress of carcinogenesis of the cervical cancer was strongly influenced by sezual hormones.

通过临床和体外研究阐明了人类乳头瘤病毒（HPV）感染的重要性。不仅在鳞状细胞癌中，而且在腺癌HPV中也以高频注意。在HPV阴性癌细胞系中观察到异常的抑制癌基p53，而在HPV阳性细胞系中仅检测到野生型p53。通过将HPV 18引入正常子宫颈的二次培养细胞，建立了新的永生细胞系。源自宫颈细胞的细胞系显示出强烈的非典型性和细胞角蛋白类似于腺癌细胞系的HeLa细胞。该细胞系将有助于研究子宫颈腺癌的致癌作用。随访了发育不良的患者。在30-40岁的患者中，经常检测到高风险HPV（16/18型）。进展的病例主要在40s中观察到。 50岁以上的患者进展或消退的病例很少。这一事实清楚地表明，宫颈癌的癌变的前期或消退受到心sasal激素的强烈影响。

项目成果

原 浩一: "子宮頸部腺癌" Oncol.Chemotherapy. 8. 19-24 (1992)

原浩一：“宫颈腺癌”Oncol.Chemotherapy。8. 19-24 (1992)

Iwasaka, T,Oh-uchida, M., Matsuo, N., Yokoyama, M., Fukuda, K., Hara, K., Fukuyama, K., Hori, K.& Sugimori, H.: "Correlation between HPV positivity and state of the p53 gene in cervical carcinoma cell lines." Gynecol Oncol. 48. 104-109 (1993)

岩坂 T、大内田 M.、松尾 N.、横山 M.、福田 K.、原 K.、福山 K.、堀 K.

Yokoyama, M., Tsutumi, K., Pater, A.& Pater, M.: "Human papillomavirus 18-immortalized endocervical cells with in vitro cytokeratin expression characteristics of adenocarcinoma." Obstet Gynecol. 83. 197-204 (1994)

横山，M.，Tsutumi，K.，佩特，A.

Hayasi,Yosinobu: "Expression of ras oncogene product und EGFreceptor in cervical squamous cell acrcinomas." Gynecol Oncol. 40. 147-151 (1991)

Hayasi，Yosinobu：“ras 癌基因产物和 EG 受体在宫颈鳞状细胞癌中的表达。”

内山 倫子: "婦人科疾患における抗PCNA抗体の細胞診への応用について" 日本臨床細胞学会雑誌. 32. 399-403 (1993)

Michiko Uchiyama：“抗 PCNA 抗体在妇科疾病细胞诊断中的应用”日本临床细胞学会杂志 32. 399-403 (1993)。