Relation of HPV Infection and Oncogenes in the Cervical Cancer.

HPV 感染与宫颈癌癌基因的关系。

基本信息

批准号：
63570788
负责人：
SUGIMORI Hajime
金额：
$ 1.28万
依托单位：
Saga Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

1) Filter hybridization for detection of HPV DNA was tried. Many methods and efforts were done in vain due to much contamination or vague reaction. Finally, Vira Pap kit, utilizing RNA probes, was recognized as good screening method for its clear cut reaction. The cervical cancer related lesions showed 59% positivity, whereas the healthy control showed only 2.5% positive.2) In situ hybridization was performed in paraffin slides of CIN patients. In CIN grade 1, 2, 3, HPV was detected in 64, 90 and 56% respectively. HPV Type 6/11 was 3%, type 16/18 was 44% and type 31/33/35 was 21%. Follow study of the patients revealed that cases with type 16/18 seemed to be higher risk for progression than cases with other types, and that cases with type 6/11 loosed their lesions during the period.3) Twenty four cases with the cervical cancer were studied. HPV DNA was detected by Southern blot technique in 25, 4 and 17% for type 16, 18 and related type respectively. When PCR technique was applied incidence of type 16 and 18 increased to 50 and 8% respectively. This result suggests that many cervical cancer have scanty HPV DNA, which scarcely detected by PCR method.4) In the cervical cancer expression of C-myc oncogene was noticed in 4%. Northern blot method revealed overexpression of C-myc RNA in 30%. No expression was noted concerning Ha-ras and erb B oncogenes.5) C-myc protein, ras P21 and EGFR were positive by immunohistochemistry in 8, 14 and 5% respectively. More frequent lymphnode metastasis was noticed in the ras-positive cancers.

1）尝试检测HPV DNA的过滤杂交。许多方法和努力是由于许多污染或模糊的反应而徒劳无功的。最后，利用RNA探针的Vira Pap套件被认为是其清晰切割反应的良好筛选方法。宫颈癌相关的病变显示59％的阳性，而健康的对照仅显示2.5％阳性。2）在CIN患者的石蜡片中进行原位杂交。在CIN 1、2、3中，HPV分别在64％，90％和56％中检测到HPV。 HPV 6/11型为3％，16/18型为44％，31/33/35型为21％。对患者进行的研究表明，患有16/18型的病例似乎比其他类型的病例更高，并且在此期间6/11型病例松开了病变。3）研究了二十四例宫颈癌。通过Southern印迹技术在25、4和17％的16、18和相关类型中通过Southern印迹技术检测到HPV DNA。当应用PCR技术的发生率分别为16和18时，分别增加到50和8％。该结果表明，许多宫颈癌的HPV DNA几乎没有通过PCR方法检测到。4）在c-myc癌基因的宫颈癌表达中，有4％的发现。 Northern印迹法显示C-Myc RNA的过表达为30％。没有关于HA-RAS和ERB B癌基因的表达。5）C-MYC蛋白，Ras P21和EGFR分别在8、14和5％的免疫组织化学中呈阳性。在RAS阳性癌症中发现了更频繁的淋巴转移。