A study for mecanism of polyclonal B cell activation in patient with cancer. (I)

癌症患者多克隆 B 细胞激活机制的研究。

• 批准号: 60570948
• 负责人: YAMAGUCHI Misao
• 金额: $ 0.26万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570948/
• 关键词: Actinomyces viscosus; Human peripheral blood lymphocytes; Flow cytometer; TCA cycle; Succinate metabolism; Immunoglobulin synthesis; General anesthesia

1. The supernatant of sonicated Actinomyces viscosus (AVsup) were examind for it's mitogenecity to human peripheral blood lymphocytes (PBL).: AVsup induced a low increase of DNA synthesis on T cell and B cell fraction, but a higher increase on unfractionated lymphocytes (UNF). AVsup did not induced of OKT3(+), OKT4(+), OKT8(+) cell population. However, it increased OKDR(+) cell population included activated T cell, 70% of B cells, 55% of monocytes, but only monocytes were excluded by appropriate gating on the cytogram of the flow cytometer, although it faild to increased OKB7(+) cell population. So the increasing of OKDR antigen positive cell population was considered not as B cells but activated T cell.2. Human PBL were cultured with succinate 1,4- <^(14)C> , which is a TCA cycle intermediated metabolic substance, in the stimulation with pokeweed mitogen (PWM). It was recognized that <^(14)C> was taken into the lymphocytes, utillized for cell metabolism and finally synthesized into im … More munoglobulins.: (1) <^(14)C> concentration in the UNF kept at low level for 1-7 days when cultured without PWM. However the stimlating with PWM increased the level by 3 times in the first day, and then they decleased with day. (2) <^(14)C> concentration in the macromolecucular substances detected in the PWM-stimulated culture sup increased with time from the third day on. The maximum value of <^(14)C> in the macromolecular substances was 0.4% of that initially added. (3) Immunoglobulins (IgG,A and M) containing <^(14)C> were detected in the sup after 7 days culture with PWM. They showed 5.6% of <^(14)C> which was detected in the macromolecular substances in the sup. The order of <^(14)C> content was B<UNF<T+B fraction.3. Infuluence of general anesthesia on the percentage of lymphocyte subset in PBL. Lymphocyte membrane markers were analysed with flow cytometry. The results demonstrated that the positive percentage of CD3, CD4, CD20 and the ratio of CD4/8 were found to be significantly lower, and that of CD8 were found to be significantly higher during anestesia than pre and postanesthesia. Less

1. 超声处理的粘性放线菌 (AVsup) 上清液检测其对人外周血淋巴细胞 (PBL) 的有丝分裂能力。：AVsup 诱导 T 细胞和 B 细胞部分 DNA 合成的低度增加，但对未分级淋巴细胞 (UNF) 的 DNA 合成增加较高。 AVsup 不诱导 OKT3(+)、OKT4(+)、OKT8(+) 细胞群。然而，它增加了OKDR(+)细胞群，包括活化的T细胞、70%的B细胞、55%的单核细胞，但通过流式细胞仪细胞图上的适当门控仅排除单核细胞，尽管它未能增加OKB7(+)细胞群。因此OKDR抗原阳性细胞群的增加不认为是B细胞，而是活化的T细胞。 2．在商陆有丝分裂原(PWM)的刺激下，用琥珀酸1,4-<^(14)C>培养人PBL，琥珀酸1,4-<^(14)C>是TCA循环中间代谢物质。人们认识到<^(14)C>被摄入淋巴细胞，用于细胞代谢，最终合成免疫球蛋白。： (1)在无PWM培养时，UNF中的<^(14)C>浓度保持在低水平1-7天。然而，利用 PWM 刺激，第一天的水平增加了 3 倍，然后随着时间的推移而下降。 (2)从第三天起，在PWM刺激的培养物sup中检测到的大分子物质中的<^(14)C>浓度随着时间的推移而增加。大分子物质中<^(14)C>的最大值为初始添加量的0.4%。 (3)用PWM培养7天后，在sup中检测到含有<^(14)C>的免疫球蛋白(IgG、A和M)。他们显示出5.6%的<^(14)C>，这是在sup中的大分子物质中检测到的。 <^(14)C>含量的顺序为B<UNF<T+B分数。3．全身麻醉对PBL中淋巴细胞亚群百分比的影响。用流式细胞术分析淋巴细胞膜标记物。结果显示，麻醉期间CD3、CD4、CD20的阳性百分率和CD4/8的比值明显低于麻醉前和麻醉后，CD8的阳性率明显高于麻醉前和麻醉后。较少的

项目成果

笹倉裕一: 日本口腔外科学会雑誌. 33(1). 32-42 (1987)

佐仓雄一：日本口腔颌面外科杂志 33(1) (1987)。

Yuuich Sasakura: Misao Yamaguchi: "Induction of human peripheral blood lymphocyte activation by supernatant of sonicated Actimomyces viscosus." "Succinate metabolism of human peripheral lymphocytes in immunoglobulin synthesis." Jpn. J. Maxillofac. Surg.Jp

Yuuich Sasakura：Misao Yamaguchi：“通过超声处理的粘性放线菌上清液诱导人外周血淋巴细胞活化。”

山口操: 日本口腔外科学会雑誌. 33(4). (1987)

山口美佐绪：日本口腔颌面外科杂志 33(4) (1987)。