A study for mecanism of polyclonal B cell activation in patient with cancer. (I)

1. 用超声法测定粘放线菌（AVsup）上清液对人外周血淋巴细胞（PBL）的有丝分裂能力。AVsup诱导T细胞和B细胞部分DNA合成的低增加，但对未分离淋巴细胞（UNF）的DNA合成增加较高。AVsup对OKT3（+）、OKT4（+）、OKT8（+）细胞群无诱导作用。然而，它增加了OKDR（+）细胞群，包括活化的T细胞，70%的B细胞，55%的单核细胞，但流式细胞仪细胞图上适当的门控只排除了单核细胞，尽管它没有增加OKB7（+）细胞群。因此，OKDR抗原阳性细胞群的增加不是B细胞，而是活化的T细胞。用琥珀酸1,4- <^(14)C> （TCA循环中间代谢物质）在美洲商陆丝裂原（PWM）刺激下培养人PBL。<^(14)C>被带入淋巴细胞，用于细胞代谢，最终合成更多的免疫球蛋白。：(1) <^（14）在不加PWM培养的情况下，UNF中C>浓度保持在低水平1-7 d。而脉宽调制刺激在第一天增加了3倍，然后随着时间的推移逐渐减弱。(2)从第3天开始，在pwm刺激培养液中检测到的大分子物质中<^(14)C>浓度随时间增加而增加。大分子物质中<^(14)c>的最大值为初始加入量的0.4%。(3)经PWM培养7 d后，血清中可检测到含有<^(14)C>的免疫球蛋白（IgG、A、M）。<^(14)C>含量的大小顺序为B<UNF<T+B分数。全麻对PBL淋巴细胞亚群百分比的影响。用流式细胞术分析淋巴细胞膜标记物。结果显示，麻醉时CD3、CD4、CD20阳性率和CD4/8比值明显低于麻醉前和麻醉后，CD8阳性率明显高于麻醉前和麻醉后。少

1. The supernatant of sonicated Actinomyces viscosus (AVsup) were examind for it's mitogenecity to human peripheral blood lymphocytes (PBL).: AVsup induced a low increase of DNA synthesis on T cell and B cell fraction, but a higher increase on unfractionated lymphocytes (UNF). AVsup did not induced of OKT3(+), OKT4(+), OKT8(+) cell population. However, it increased OKDR(+) cell population included activated T cell, 70% of B cells, 55% of monocytes, but only monocytes were excluded by appropriate gating on the cytogram of the flow cytometer, although it faild to increased OKB7(+) cell population. So the increasing of OKDR antigen positive cell population was considered not as B cells but activated T cell.2. Human PBL were cultured with succinate 1,4- <^(14)C> , which is a TCA cycle intermediated metabolic substance, in the stimulation with pokeweed mitogen (PWM). It was recognized that <^(14)C> was taken into the lymphocytes, utillized for cell metabolism and finally synthesized into im … More munoglobulins.: (1) <^(14)C> concentration in the UNF kept at low level for 1-7 days when cultured without PWM. However the stimlating with PWM increased the level by 3 times in the first day, and then they decleased with day. (2) <^(14)C> concentration in the macromolecucular substances detected in the PWM-stimulated culture sup increased with time from the third day on. The maximum value of <^(14)C> in the macromolecular substances was 0.4% of that initially added. (3) Immunoglobulins (IgG,A and M) containing <^(14)C> were detected in the sup after 7 days culture with PWM. They showed 5.6% of <^(14)C> which was detected in the macromolecular substances in the sup. The order of <^(14)C> content was B<UNF<T+B fraction.3. Infuluence of general anesthesia on the percentage of lymphocyte subset in PBL. Lymphocyte membrane markers were analysed with flow cytometry. The results demonstrated that the positive percentage of CD3, CD4, CD20 and the ratio of CD4/8 were found to be significantly lower, and that of CD8 were found to be significantly higher during anestesia than pre and postanesthesia. Less