A study for mecanism of polyclonal B cell activation in patient with cancer. (I)

1.本文研究了粘性放线菌（Actinomycesviscosus，AVsup）超声培养上清对人外周血淋巴细胞（PBL）的促分裂活性。AVsup对T细胞和B细胞DNA合成的促进作用较弱，但对未分级淋巴细胞（UNF）DNA合成的促进作用较强。AVsup对OKT 3（+）、OKT 4（+）、OKT 8（+）细胞群无诱导作用。OKDR（+）细胞数增加，其中包括活化的T细胞、70%的B细胞和55%的单核细胞，但在流式细胞仪的细胞图上通过适当的门控仅排除单核细胞，但不能增加OKB7（+）细胞数。因此认为OKDR抗原阳性细胞数量的增加不是B细胞的作用，而是活化的T细胞的作用.在商陆有丝分裂原（PWM）刺激下，将人PBL与TCA循环中间代谢物质琥珀酸1，4-<^（14）C>共同培养。人们认识到，<^（14）C>被吸收到淋巴细胞中，用于细胞代谢，最后合成为免疫球蛋白。 ...更多信息 muneulins.：(1)无PWM时，UNF中<^（14）C>浓度维持在低水平1 - 7天。而PWM刺激第一天使其水平增加3倍，随后随时间下降。(2)从第3天开始，在PWM刺激的培养液中检测到的大分子物质中<^（14）C>浓度随时间增加，最大值为初始加入量的0.4%。(3)PWM培养7天后，在上清液中检测到含<^（14）C>的免疫球蛋白（IgG、A和M）。它们显示出5.6%的<^（14）C>，这是在sup中的大分子物质中检测到的。<^（14）C>含量顺序为B <UNF <T + B.全麻对外周血淋巴细胞亚群的影响。用流式细胞术分析淋巴细胞膜标志物。结果表明：麻醉期间CD_3、CD_4、CD_（20）阳性率及CD_（4/8）比值明显低于麻醉前和麻醉后，CD_（8）阳性率明显高于麻醉前和麻醉后。少

1. The supernatant of sonicated Actinomyces viscosus (AVsup) were examind for it's mitogenecity to human peripheral blood lymphocytes (PBL).: AVsup induced a low increase of DNA synthesis on T cell and B cell fraction, but a higher increase on unfractionated lymphocytes (UNF). AVsup did not induced of OKT3(+), OKT4(+), OKT8(+) cell population. However, it increased OKDR(+) cell population included activated T cell, 70% of B cells, 55% of monocytes, but only monocytes were excluded by appropriate gating on the cytogram of the flow cytometer, although it faild to increased OKB7(+) cell population. So the increasing of OKDR antigen positive cell population was considered not as B cells but activated T cell.2. Human PBL were cultured with succinate 1,4- <^(14)C> , which is a TCA cycle intermediated metabolic substance, in the stimulation with pokeweed mitogen (PWM). It was recognized that <^(14)C> was taken into the lymphocytes, utillized for cell metabolism and finally synthesized into im … More munoglobulins.: (1) <^(14)C> concentration in the UNF kept at low level for 1-7 days when cultured without PWM. However the stimlating with PWM increased the level by 3 times in the first day, and then they decleased with day. (2) <^(14)C> concentration in the macromolecucular substances detected in the PWM-stimulated culture sup increased with time from the third day on. The maximum value of <^(14)C> in the macromolecular substances was 0.4% of that initially added. (3) Immunoglobulins (IgG,A and M) containing <^(14)C> were detected in the sup after 7 days culture with PWM. They showed 5.6% of <^(14)C> which was detected in the macromolecular substances in the sup. The order of <^(14)C> content was B<UNF<T+B fraction.3. Infuluence of general anesthesia on the percentage of lymphocyte subset in PBL. Lymphocyte membrane markers were analysed with flow cytometry. The results demonstrated that the positive percentage of CD3, CD4, CD20 and the ratio of CD4/8 were found to be significantly lower, and that of CD8 were found to be significantly higher during anestesia than pre and postanesthesia. Less