权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Investigation of Celluar Movements by Magetometry and Microscopic Picture Processing

通过测磁和显微图像处理研究细胞运动

基本信息

批准号：
61550310
负责人：
NEMOTO Iku
金额：
$ 1.09万
依托单位：
Tokyo Denki University, Faculty of Science and Engineering
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61550310/
关键词：
biomagnetism cellula motility macrophages image processing 光学顕微鏡 ATP 顕微鏡画像処理

项目摘要

Intracelluar motility of cells (hamster alveolar macrophages) have been studied by magnetometry and by microscopic image processing. Ferrimagnetic particles were introduced into the macro phages through phabocytosis. The particles, when aligned in one direction by an external field, produce a weak remanent field (RMF) around the cells. The RMF decays with time (relaxation) due to the misaligement of the organelles containing the particles. The randomization of the orientation of the partiles is thus caused by intracelluar movements of organelles and filamentous structures within the cell. Methods have been developed to investigate the intracelluar motility and physical properties of the cytoplasm by this magnetic measurements. The results so far obtained yield the relationship between the intracellualr concentration of ATP and the energy responsible for the relaxation of RMF. The effects of temperature was also studied. The results suggest that the method can be a good probe for studying intracelluar activities of cells in intact situations. We especially hope that this method may be utilized to investigate the mechanisms in which the hydrolysis of ATP moves the filaments. Microscopic image processing was studied to obtain clearer images of magnetic particles within the cells than avaliable by direct obaservaytion or photography of microscopic images. Our approach is to interage the chatacteristics of the optical system (microscope) with computer processing of the images. For the puepose, basic research of the three-dimensional properties of microscopic imaging was done ad well as implenetation of three-dimensional deconbolution. Althught the orginal purpose of this study was within a rather narrow angle, the results we have obtained anf well obtain in the neasr future are more basic and general than we had anticipated. Therefore, we will continue this part of the research more of less independently of the motility sutdies of the cell.

细胞（仓鼠肺泡巨噬细胞）的细胞内运动已被研究的磁力和显微图像处理。通过吞噬作用将铁磁性颗粒引入到巨噬细胞中。当粒子在外场作用下沿一个方向排列时，在细胞周围产生弱反射场（RMF）。由于含有颗粒的细胞器的错位，RMF随时间衰减（弛豫）。因此，颗粒取向的随机化是由细胞器和细胞内丝状结构的细胞内运动引起的。通过这种磁测量，已经发展了研究细胞内运动和细胞质物理性质的方法。到目前为止所获得的结果产生的关系intracellualr ATP浓度和能量负责RMF的松弛。还研究了温度的影响。结果表明，该方法可作为研究完整状态下细胞内活动的良好探针。我们特别希望这种方法可以用来研究ATP水解移动细丝的机制。研究了显微图像处理，以获得比直接观察或显微图像摄影更清晰的细胞内磁性颗粒的图像。我们的方法是将光学系统（显微镜）的特性与计算机图像处理相结合。为此，对显微成像的三维特性进行了基础研究，并实现了三维解卷积。尽管本研究的初衷是在一个相当狭窄的角度内进行的，但我们所得到的结果以及在不久的将来所能得到的结果比我们预期的要更基本、更普遍。因此，我们将继续这部分的研究，或多或少独立于细胞的运动研究。