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Investigation of interactions between the organelle motion and filamentous structures within cells by cytomatomagnetometry.

通过细胞磁力测量研究细胞内细胞器运动和丝状结构之间的相互作用。

基本信息

批准号：
04452212
负责人：
NEMOTO Iku
金额：
$ 1.73万
依托单位：
Tokyo Denki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

We studied the feasibility and efficacy of a mangetometric method for investigations of intracellular motions, particularly the possible interactions between phagosome membrane and filamentous strucures within the cell. The present investigation has focued on showing that this method can be a good probe in investiating the elementary processes in phagosome motions. Cytomagnetometry, as we call it, has the following advantages. 1)Intracellular motions within living cells can be measured ; 2)Measurement itself is relatively easy ; 3)Magnetic particles can be manipulated by an external force non-invasively and thus can be subject to a large variety of experiments ; 4)The measurement is done on a large number of cells at a time, resulting in enough averaging.It has now become clear that cytomagnetometry yields data which reflect intracellular motions of phagosomes and by manipulating the magnetic particles within cells one can obtain data which also reflect the rheological properties.Our experiments focused upon the hypothesis of phagosome motion through interaction of actinfilaments and myosin on the phagosome membrane. The results show that actin filaments do play some role in phagosme motion, although the results were not as clear cut and decisive as we had hoped. Experiments performed to see viscocity generation by actin filaments yielded intriguing but interesting results which further suggest actin's involvement in phagosome motions.We also built a mathematical model in which phagosome motions are caused by association of myosin on the phagosome membrane with an actin filament surrounding the phagosome and its decomposition caused by ATP hydrolysis. Association and decomposition were assumed to be Poisson processes and it was shown that it caused a rotationary random walk and also that the association acts as friction to external forces.

我们研究了磁测量法用于研究细胞内运动的可行性和有效性，特别是吞噬体膜和细胞内丝状结构之间可能的相互作用。本研究的重点是表明，这种方法可以作为研究吞噬体运动基本过程的一种很好的探索。我们称之为细胞磁性测定法，具有以下优点。1)可以测量活细胞内的细胞内运动; 2）测量本身相对容易; 3）磁性颗粒可以通过外力非侵入性地操纵，因此可以进行各种各样的实验; 4）一次对大量小区进行测量，现在已经清楚的是，细胞磁性测量产生的数据反映了吞噬体的细胞内运动，并通过操纵磁性颗粒，在细胞内，我们可以获得同样反映流变学特性的数据，我们的实验集中在通过吞噬体膜上的肌动蛋白丝和肌球蛋白的相互作用来进行吞噬体运动的假设上。结果表明，肌动蛋白丝确实在吞噬体运动中起一定作用，尽管结果并不像我们所希望的那样明确和决定性。通过实验观察肌动蛋白丝产生粘性的过程，得到了有趣的结果，进一步表明肌动蛋白参与了吞噬体的运动，我们还建立了一个数学模型，其中吞噬体运动是由吞噬体膜上的肌球蛋白与围绕吞噬体的肌动蛋白丝的结合以及ATP水解引起的分解引起的。结合和分解被假定为泊松过程，它被证明是一个旋转的随机行走，也是作为摩擦的关联外力。