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Quantitative investigation of the dynamics and mechanical properties of cytoskeleton through cytomagnetometric and optical measurement of living cells.

通过活细胞的细胞磁力测量和光学测量来定量研究细胞骨架的动力学和机械特性。

基本信息

批准号：
08458294
负责人：
NEMOTO Iku
金额：
$ 2.5万
依托单位：
Tokyo Denki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08458294/
关键词：
cellular motility cytoskeleton macrophage biomagnetism cytomagnetometry phagosome microfilaments microtubules 細胞磁気測定

项目摘要

Cytomagnetometry is the measurement of the magnetic field from the cells which have engulfed magnetic particles. The particles can be manipulated by an external field and hencecan be used as a probe to investigate the mechanisms of the intracellular movements and the mechanical properties of the tissues surrounding the phagosomes. After magnetizing the particles within the cells, the cell field decays due to the random motion of the phagosmes. Therefore, the cell field can be related to the energy responsible for the random motion and to the rheological properties of the tissues. In this particular research, we have focused on the viscoelasticity of the tissues. When the phagosomes containing magnetized particles are given a torque by an external field, the cell field decays quickly, but when the torque is released, the cell field shows a recoil which is ascribed to some elasticity. We have modeled the situation by a simple differential eqution with two viscous and one elastic components. This equation is driven by the Wiener process. We have mode Monte Carlo simulations to calculate the cell field from this equation. We also have derived the partial differential equation describing the time course of the probabillty density of the orientation of the phagosomes. Experimentally, we have used drugs to destroy filamentous structures such as microfilaments and microtubules. The behavior of the cell field after treatment by these drugs suggest complicated effects of these drugs to the cytoskeletal structures surrounding the phagosomes but further investigation is necessary to compare the results with the numerical experiments of the model. Optical study using the confocal laser scanning microscope has been started but has not produced enough results to be compared with the magnetic study.

细胞磁性测定法是对吞噬了磁性颗粒的细胞产生的磁场进行测量。这种颗粒可以被外场操纵，因此可以作为探针来研究吞噬体周围组织的机械性质和细胞内运动的机制。在磁化细胞内的粒子之后，由于吞噬体的随机运动，细胞场衰减。因此，细胞场可以与随机运动的能量以及组织的流变学特性相关。在这个特殊的研究中，我们集中在组织的粘弹性。当含有磁化颗粒的吞噬体受到外场的扭矩时，细胞场迅速衰减，但当扭矩释放时，细胞场表现出一定弹性的反冲。我们已经模拟了一个简单的微分方程的情况下，两个粘性和一个弹性组件。该方程由维纳过程驱动。我们有模式蒙特卡罗模拟计算细胞场从这个方程。我们还导出了描述吞噬体取向概率密度随时间变化的偏微分方程。在实验中，我们已经使用药物来破坏丝状结构，如微丝和微管。这些药物治疗后的细胞场的行为表明这些药物对吞噬体周围的细胞骨架结构的复杂影响，但需要进一步的研究来比较模型的数值实验结果。使用共聚焦激光扫描显微镜的光学研究已经开始，但尚未产生足够的结果与磁性研究进行比较。