Approaches to vaccines against bovine Theileriosis

在T.Sergenti的生命周期中，孢子虫、精神分裂症和Merozoids的形态学变化被观察到作为疫苗来源使用，我们试图生产对抗T.Sergenti每个阶段的单克隆抗体。Merozoites。13种单克隆抗体对T.sergenti蛋白质的分析做好了准备。所有单克隆抗体都被固定在piroplasm中，但没有被间接荧光抗体测试感染的细胞。Western blot分析显示了6个混合体测试的重新确定的palypeptides，分子重量为32，000或23，000。这些抗体通过免疫电子显微观测与美佐托细胞的细胞膜反应，但没有与受感染的红细胞的面纱和屏障结构反应。一套内神经细胞学多重合成法被标记为rhopties、microneme和inner membrane的外观。有人提出了一种在体外细胞化中划分的四种形式，即美罗佐石具有常见的无atigens和易感性，因为美罗佐石具有相同的结构性特征。孢子虫。在矢量的光芒中，在替换后的48小时内出现了宝石。在被感染的成人和nymph ticks的Salivary gland被保留在25 ° C，被诱导在30天回复后喂养兔子的ticks。孢子虫的感染水平比成人的乳头高。在盐水土地形成的合成细胞中被注射到痤疮，并由布丁生产了超过1000种孢子虫。我们试着准备一种对抗孢子虫的单抗抗体。Schizonts， 15只蝴蝶是一种未饱和的孢子虫，可以在淋巴细胞中检测出精神分裂症，像Protozoa只在5个病例中观察到。Althouht Attenuated schizontvaccine被报道为T.annulata，T.sergenti的状态没有被收获足够的体积用于一项免疫学研究。

Morphological changes of sporozoites, schizonts and merozoites in the life cycle of T.sergenti were observed to use as the vaccine sources, we attempted to produce monoclonal antibodies against each stage of T.sergent.1. Merozoites. Thirteen monoclonal antibodies against merozoites of T.sergenti were prepared for analysis of their structural proteins. All monoclonal antibodies stained the piroplasm, but not aninfected erythrocytes by indirect fluorescent antibody test. Western blot analysis showed that 6 hybridomas tested recongnized palypeptides with molecular weights of 32,000 or 23,000. These antibodies reacted with cell membrane of merozoites by immuno electron microscopical observations, but did not react with veil and bar-structure of infected erythrocytes. The onset of intraerythrocytic multiplication was marked by the appearance of rhopties, microneme and inner membrane. It was suggested that four dividing forms in intraerythrocytic merozoites had common anatigens and infective ability, because this merozoite had the same instructural features as the sporozoites.2. Sporozoites. In the lumen of the gut of vector ticks, gametocytes appeared with in 48 hours after repletion. The sporozoites in the salivary glands of infected adult and nymph ticks maintained at 25゜C were induced by feeding ticks on rabbit after 30 days repletion. Infection level of sporozoites was high in nymph than adult ticks. A kinete penetrated to acini in the salivary glands formed syncytium and it produced over ten thousand sporozoites by budding. We tried to prepared monolonal antibody against the sporozoites.3. Schizonts, 15 cattle were inoculated sporozoites to detect schizonts in lymphocytes, schizont like protozoa were observed only in 5 cases. Althouht attenuated schizont vaccine for T.annulata were reported, shizonts of T.sergenti were not harvested sufficient volume to use for a immunoligical studies.