Disturbance of Nitrogen Metabolism in sparse-fur mice.

稀疏毛皮小鼠氮代谢紊乱。

基本信息

  • 批准号:
    61570144
  • 负责人:
  • 金额:
    $ 1.09万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1986
  • 资助国家:
    日本
  • 起止时间:
    1986 至 1987
  • 项目状态:
    已结题

项目摘要

In the present study, nitrogen metabolism of sparse-fur (spf). or ornithine transcarbamylase (OTC)-deficient mice was investigated. Spf mice have kinetically abnormal OTC in the liver and intestine,which would cause hyperammonemia and arginine deficiency. The latter was true; they showed low citrulline and arginine concentrations in the serum and in the brain always at the young and old ages. Serum ammonia concentration of spf mice, however, was not elevated at the adult ages. Only during the weaning period for two to three weeks, they showed elevated serum ammonia levels. Considering these results, we compared nitrogen metabolism of spf mice between hyperammonemic stage (30 to 45 days old) and adapted stage (after 50 days old). At hyperammonemic stage, ammonia concentration was elevated in the brain and the level was correlated with the concentrations of glutamine in the brain. And the latter level was corelated with the concentrations of phenylalanine, tyrosine and tryptophan. At this stage,metabolism of 5-hydroxytryptamine was stimulated, as judged from the higher ratio of 5-hydroxytryptamine to 5-hyroxyindole acetic acid. This may cause some neurological symptoms of hyperammonemia. At both stages, levels of citrulline and arginine in the brain were very low. In order to test the ability of ammonia detoxication in the spf mice, liver perfusion experiments were performed. Spf mice showed the lower activity of urea formation from ammonium salt especially under no addition of ornithine. which may be indicative of abnormal kinetic properties of OTC in spf mice. Urinary orotic acid concentration was very high at both stages and is again shown to be a good marker for OTC deficiency.
本文研究了稀毛雀的氮代谢。或鸟氨酸转氨甲酰酶(OTC)缺陷小鼠。Spf小鼠的肝脏和肠道中存在动力学异常的OTC,这会导致高氨血症和精氨酸缺乏。后者是正确的;他们在年轻和老年时血清和大脑中的瓜氨酸和精氨酸浓度都很低。而spf小鼠的血清氨浓度在成年期并没有升高。只有在断奶期间的两到三个星期,他们表现出血清氨水平升高。考虑到这些结果,我们比较了spf小鼠在高氨血症阶段(30 - 45日龄)和适应阶段(50日龄后)的氮代谢。在高氨血症阶段,脑内氨浓度升高,其水平与脑内谷氨酰胺浓度相关。后者与苯丙氨酸、酪氨酸和色氨酸的浓度相关。在此阶段,5-羟色胺的代谢受到刺激,根据5-羟色胺与5-羟吲哚乙酸的较高比率判断。这可能会导致高氨血症的一些神经症状。在这两个阶段,大脑中的瓜氨酸和精氨酸水平都非常低。为了检测spf小鼠对氨的解毒能力,进行了肝脏灌流实验。Spf小鼠表现出较低的由铵盐生成尿素的活性,尤其是在不添加鸟氨酸的情况下。这可能表明OTC在SPF小鼠中的异常动力学性质。两个阶段的尿乳清酸浓度都很高,再次证明是OTC缺乏的良好标志物。

项目成果

期刊论文数量(8)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ituro Inoue.: "Accumulation of large neutral amino acid in the brain of sparse-fur mice at hyperammonemic state." Biochemical Medicine and Metabolic Biology. 38. 378-386 (1987)
Ituro Inoue.:“高氨状态下稀疏毛皮小鼠大脑中大量中性氨基酸的积累。”
  • DOI:
  • 发表时间:
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  • 影响因子:
    0
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  • 通讯作者:
井ノ上逸朗: 鹿児島大学医学部附属動物実験施設年報. 11. 11-19 (1986)
Ituro Inoue:鹿儿岛大学医学院附属动物实验设施的年度报告。11. 11-19 (1986)。
  • DOI:
  • 发表时间:
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    0
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  • 通讯作者:
井ノ上逸朗: Biochmical Medicine and Metabolic Biology. 38. 378-386 (1987)
Ituro Inoue:生物化学医学和代谢生物学。38. 378-386 (1987)
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    0
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  • 通讯作者:
具志堅隆: Biochmistry International. 11. 637-643 (1985)
固志隆:国际生物化学。11. 637-643 (1985)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Takashi Gushiken.: "Transient hyperammonemia during aging in ornithine transcarbamylase-deficient, sparse-fur mice." Biochemisty International. 11. 637-643 (1985)
Takashi Gushiken.:“鸟氨酸转氨甲酰酶缺陷、毛发稀疏的小鼠在衰老过程中出现短暂的高氨血症。”
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  • 影响因子:
    0
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SAHEKI Takeyori其他文献

SAHEKI Takeyori的其他文献

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{{ truncateString('SAHEKI Takeyori', 18)}}的其他基金

To developtherapeutic and prophylactic procedures for citrin deficiency
开发柑橘缺乏症的治疗和预防程序
  • 批准号:
    24591516
  • 财政年份:
    2012
  • 资助金额:
    $ 1.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Toxicity of sugars and identification of markers for pathophysiology in citrin deficiency leading to development of novel therapeutics
糖的毒性和柑橘缺乏症病理生理学标志物的鉴定导致新疗法的开发
  • 批准号:
    21591337
  • 财政年份:
    2009
  • 资助金额:
    $ 1.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Pathophysiological analysis and development of therapeutic procedures for citrin deficiency by using a mouse model
使用小鼠模型进行柑橘缺乏症的病理生理学分析和治疗方法的开发
  • 批准号:
    19591230
  • 财政年份:
    2007
  • 资助金额:
    $ 1.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Structure and function of novel genes which differentially expressed in cardiac hypertrophy of carnitine deficiency
肉碱缺乏性心脏肥大差异表达新基因的结构和功能
  • 批准号:
    10470042
  • 财政年份:
    1998
  • 资助金额:
    $ 1.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Mechanism of suppression of urea cycle enzyme gene expression under carnitine deficiency in juvenile visceral steatosis mice.
肉碱缺乏下幼年内脏脂肪变性小鼠尿素循环酶基因表达抑制机制。
  • 批准号:
    08670182
  • 财政年份:
    1996
  • 资助金额:
    $ 1.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Pathogenesis and pathophysiology of inherited carnitine deficiency using carnitine-deficient JVS mice
使用肉碱缺陷 JVS 小鼠遗传性肉碱缺乏的发病机制和病理生理学
  • 批准号:
    05454170
  • 财政年份:
    1993
  • 资助金额:
    $ 1.09万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Argininosuccinate as a possible neuromodulator and localization of its metabolizing enzymes
精氨基琥珀酸作为一种可能的神经调节剂及其代谢酶的定位
  • 批准号:
    03807012
  • 财政年份:
    1991
  • 资助金额:
    $ 1.09万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Possible involvement of antisense RNA in pathogenesis of type II citrullinemia.
反义 RNA 可能参与 II 型瓜氨酸血症的发病机制。
  • 批准号:
    62570120
  • 财政年份:
    1987
  • 资助金额:
    $ 1.09万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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