Structure and function of cell membrane associated with multidirectional differentiation of lymphatic leukemia

淋巴白血病多向分化相关细胞膜的结构和功能

• 批准号: 61570172
• 负责人: MAEDA Sakan
• 金额: $ 1.22万
• 依托单位: Kobe University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570172/
• 关键词: Cell differentiation; Stem cell; Cell membrane; Suger chain; Acute lymphoblastic leukemia; Adult T-cell leukemia; Chromosome abnormalities; プロモーター; 培養株の樹立

Many reports indicate that plasma membrane glycoproteins may be associated with various kinds of cellular recgnition as receptors. Our previous studies on cell surface glycoproteins of different stage of human and rat leukemia cell lines indicated that the structural information on surface glycoproteins and asparagine-linked sugar chain patterns was essential to understand the biological role of them, and might be useful for the classification of leukemia. For the precise analysis of cell surface, the cultured leukemia cell lines might be useful because the homogeneous cell population could be obtained everytime. KH-3A,3B and KH-4 were established from 11-year-old girl and 14-year-old boy with acute lymphoblastic leukemia(ALL). Five subclones isolated from KH-3A by agarose could be induced to have T or B cell characteristics by TPA. Although KH-4 has the characteristics of common ALL, KH-3A subclones might be originated from more immature stages.The cell surface glycoproteins and asapragine-linked sugar chain patterns of ATL cell line(KH-2)were studied by using SDS-polyacrylamide gel electrophoresis and hydrazinolysis. Further analysis of KH-3a may be necessary to know the surface changes of cell differentiation

许多研究表明，细胞膜糖蛋白作为受体与多种细胞受体有关。我们以前对人和大鼠白血病细胞系不同阶段细胞表面糖蛋白的研究表明，表面糖蛋白和天冬酰胺连接糖链模式的结构信息对于理解它们的生物学作用是必不可少的，并且可能有助于白血病的分类。培养的白血病细胞系由于每次都能获得均一的细胞群体，因此对于细胞表面的精确分析是有用的。KH-3A、3B和KH-4是从11岁女孩和14岁男孩的急性淋巴细胞白血病（ALL）中建立的。经琼脂糖凝胶电泳分离的KH-3A中的5个亚克隆经TPA诱导后均具有T或B细胞特征。本文用SDS-聚丙烯酰胺凝胶电泳和肼解法研究了ATL细胞系（KH-2）的细胞表面糖蛋白和天冬酰胺连接糖链模式。进一步分析KH-3a可能是了解细胞分化表面变化的必要条件

项目成果

Kazuo Gohji: Cancer Research. 46. 6264-6268 (1986)

Kazuo Gohji：癌症研究。

H.Drexler,G.Gasedicke,S.Maeda,P.Chen and J.Minowada: "Monocytic leukemia cell line CTV-1:Morphological,Immunological and isoenzymatic characteristics." Tumor Immunology. 6. 503-517 (1986)

H.Drexler、G.Gasedicke、S.Maeda、P.Chen 和 J.Minowada：“单核细胞白血病细胞系 CTV-1：形态学、免疫学和同工酶特征。”

H,Drexlar;G.Gaedicke;S.Maeda;P.Chen;J.Minowada: Tumour Biology. 6. 503-517 (1986)

H，Drexlar；G.Gaedicke；S.Maeda；P.Chen；J.Minowada：肿瘤生物学。

Y.Nakao;T.Koizumi;T.Matsui;T.Nakagawa;Y.Katakami;T.Fijita;S.Maeda;S.Morikawa;Y.Ito: Journal of National Cancer Institute. 78. 1079-1086 (1987)

Y.Nakao；T.Koizumi；T.Matsui；T.Nakakawa；Y.Katakami；T.Fijita；S.Maeda；S.Morikawa；Y.Ito：国家癌症研究所杂志。

Miharu Nagasaka-Yabe: Japanese Journal of Cancer Research(Gann).

Miharu Nagasaka-Yabe：日本癌症研究杂志（江恩）。