Establishment of recipient cells for oncogene detection and analysis of transformation-associated cell surface antigen expression

宿主的抗肿瘤监测机制的细节仍然是未知的。NK细胞的表面表型和个体发生已被广泛研究，并且NK细胞群体之间存在相当大的异质性。然而，关于NK细胞识别的肿瘤细胞的靶结构知之甚少。我们已经证明了两种不同的候选NK靶分子，即86 kd和62 kd的蛋白质组成的一个单一的多肽链。这些分别由单克隆抗体（mabs）#109和#061定义，并且与活化的H-ras癌基因诱导的WKA大鼠胎儿来源的成纤维细胞WFB转化高度相关。这也强烈表明，86 kd的表达，而不是62 kd的分子被增强的非致瘤性WFD细胞由PDGF刺激。这些数据表明，这两种不同的分子作为靶结构直接参与NK-靶细胞相互作用。另一株识别67 kd单肽的单抗067则选择性地与W31、W14、EKras-WFB和PyMt-WFB转化子及亲本WFB发生反应，但不与W14、EKras-WFB和PyMt-WFB转化子发生反应。该抗原的表达增强W31通过热，超氧化物和cAMP或霍乱毒素处理。此外，在常规培养条件下不组成性显示该抗原的W14和其它EKras WFB转化体上通过这些处理诱导。提示该抗原是一种新的热诱导分子，可能是WFB细胞的肿瘤特异性抗原。

The detail of anti-tumor surveillance mechanisms by the hosts is still unknown. Surface phenotypes and the ontogeny of NK cells have been widely studied, and there is considerable heterogeneity among NK cell populations. However, little is known about the target structures of tumor cells that are recognized by NK cells. It is also essential to investigate how these structures could be associated with the cell transformation process.We have demonstrated two different candidates of the NK target molecules, namely 86 kd and 62 kd protein composed of a single polypeptide chain. These were defined by monoclonal antibodies (mabs), #109 and #061, respectively, and were highly associated with the activated H-ras oncogene-induced transformation of WKA rat fetus-derived fibroblast WFB. It is also strongly suggested that the expression of 86 kd and not that of the 62 kd molecule was enhanced on nontumorigenic WFD cells which were stimulated by PDGF. The data suggest that these two different molecules are directly involved in the NK-target cell interactions as the target strucutres. These molecules may play an important role in the antitumor surveillance by the hosts.On the other hand, another mab #067 recognizing 67 kd single polypeptide reacted selectively with W31 but not with w14 and other EKras-WFB or PyMt-WFB transformants as well as parental WFB. The expression of this antigen was enhanced on W31 by treatment with heat, superoxide and cAMP or cholera toxin. Furthermore, it was induced by these treatment on W14 and other EKras WFB transformants that did not show constitutively this antigen under the regular culture conditions. These data suggested that this antigen is a novel heat-inducible molecule, and might be a tumor specific antigen of WFB cells.