HLA-A31-restricted gastric tumor antigenic peptide and clinical application

HLA-A31限制性胃肿瘤抗原肽及其临床应用

• 批准号: 09557019
• 负责人: SATO Noriyuki
• 金额: $ 6.02万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557019/
• 关键词: CTL; gastric tumor; HLA-A31; peptide; cancer vaccine; 抗原ペプチド; 癌ワクチン

1 HLA-A31-restricted antigenic peptide F4.2 (YSWMDISCWI) was identified by using human gastric tumor line HST-2 and autologous cytotoxic T lymphocyte (CTL) clone TcHST-2.2 F4.2 peptide could induce in vitro peptide-specific cytotoxic T lymphocytes (CTL) in approximately one third of HLA-A31 (+) gastric cancer patients.3 We cloned a candidate of cDNA C98 that encodes F4.2 peptide parental protein. C98 is a new gene, and is composed of 537 nucleotides with the open reading frame. However, C98 encodes YSWMDIITIC rather than YSWMDISCWI in its sequence. We are now underway to investigate how YSWMDIITIC was generated in the cells.

1 HLA-A31限制性抗原肽F4.2用人胃癌细胞株HST-2和自体细胞毒性T淋巴细胞（CTL）克隆TcHST-2.2鉴定出一株特异性的细胞毒T淋巴细胞（CTL）融合蛋白（YSWMDISCWI）。3.我们克隆了一个编码F4.2肽亲本蛋白的候选cDNA C98。C98是一个新基因，由537个核苷酸组成，具有开放的阅读框架。然而，C98在其序列中编码YSWMDIITIC而不是YSWMDISCWI。我们现在正在研究YSWMDIITIC是如何在细胞中产生的。

项目成果

Kikuchi, K., and Sato, N.: "Cell-mediated Immunity to autologous cancer. Rationale of immunotherapy of human cancer with CTL.Gann Monogr.Japa.J.Cancer Res., In "Recent Advances of Human Tumor Immunology and Immunotherapy"" Gann Monogr.Jap.J.Cancer Res., E

Kikuchi, K. 和 Sato, N.：“细胞介导的自体癌症免疫。CTL 人类癌症免疫治疗的基本原理。Gann Monogr.Japa.J.Cancer Res.，在“人类肿瘤免疫学和免疫治疗的最新进展”中

Kamiguchi,K.,Sato,N.,Kikuchi,K.et al: "73 kD heat shock cognate protein is associated with transporter associated with antigen processing." Int.Immunol.(in press).

Kamiguchi,K.、Sato,N.、Kikuchi,K.et al：“73 kD 热休克同源蛋白与抗原加工相关的转运蛋白有关。”

Akazawa,T., Torigoe,T., Nagasawa,S., Tamura,Y., Sato,N.et al.: "A novel negative regular Cho-1 molecule is involved in the cytotoxicity by human natural killer cells nut not in cytotoxicity T lymphocytes." Microbiol.Immunol.in press.

Akazawa,T.、Torigoe,T.、Nagasawa,S.、Tamura,Y.、Sato,N.等人：“一种新型负规则 Cho-1 分子参与人类自然杀伤细胞的细胞毒性，但不涉及

佐原弘益,佐藤昇志,高橋延昭,菊地浩吉: "Annual Review免疫1998.中外医学社" 癌拒絶抗原ペプチドによる治療, 10 (1998)

Hiromasu Sahara、Noboru Sato、Nobuaki Takahashi、Hiroyoshi Kikuchi：“免疫学年度评论 1998。中外医学社”癌症排斥抗原肽治疗，10 (1998)

Sato, N.and Torigoe, T.: "The molecular chaperones in cell cycle control. Stress of life - from molecules to man." Annals of New York Acad.Sci.851. 61-66 (1998)

Sato, N. 和 Torigoe, T.：“细胞周期控制中的分子伴侣。生命的压力 - 从分子到人。”