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Forensic studies on genetic markers in human urine

人类尿液中遗传标记的法医研究

基本信息

批准号：
61570288
负责人：
KISHI Koichiro
金额：
$ 1.28万
依托单位：
Fukui Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

There are many usefull genetic markers in human urine. However, these markers have not yet enough used in forensic science-field. So this work was designed in order to have a wide variety of applications in this field for these markers.Pepsinogen A (PGA) was purified from human urine by a series of column chromatographies. After isoelectric focusing electrophoresis on a polyacrylamide gel (IEF-PAGE), urinary PGA isozymogens could be clearly detected by means of immunostaining with the PGA-specific antibody. PGA was generally separable into five fractions, I-V, on the gel. Fraction V was composed of F and/or S band(s). The population frequencies of three patterns of fraction V (F, FS and S) and family studies indicatied that PGA V is controlled by a pair of alleles, PGAV^*F and PAGV^*S, at a single autosomal locus, and that both are codominant.One of the human urinary ribonucleases (RNases) was isolated and purified by means of a series of column chromatographies. The enzyme was named RNase 1. Rabbit antibody to the purified RNase 1 reacted with human urine and sera, as well as the purified enzyme. The genetic polymorphism of serum RNase 1 was studied by IEF-PAGE, followed by immunostaining using antibody specific for RNase 1. Two common phenotypes, RNASE1 1 and RNASE1 1-2, were easily recognized. However, the other homogeneous phenotypes, RNASE1 2, was not detected in our samples. Family studies are in agreement with an autosomal codominant transmission of the two alleles.

人类尿液中有许多有用的遗传标记。然而，这些标记物在法医学领域的应用还不够。本研究采用柱层析法从人尿中分离纯化胃蛋白酶原A（Pepsinogen A，PGA）。在聚丙烯酰胺凝胶等电聚焦电泳（IEF-PAGE）后，尿PGA同工酶可以清楚地检测通过免疫染色与PGA特异性抗体。PGA在凝胶上通常可分离成I-V五个级分。组分V由F和/或S带组成。FHV的三种类型（F、FS和S）的群体频率和家系调查表明，PGV受一对等位基因PGAV^*F和PAGV^*S控制，且两者为共显性。这种酶被命名为RNase 1。兔抗纯化的RNase 1抗体与人尿和血清以及纯化的酶反应。采用等电聚焦聚丙烯酰胺凝胶电泳（IEF-PAGE）和特异性抗体免疫组化染色法研究血清RNase 1的遗传多态性。两种常见的表型RNASE 11和RNASE 11 -2容易识别。然而，其他同质表型，RNASE 1 - 2，在我们的样品中未检测到。家系研究结果与常染色体共显性遗传的两个等位基因。