Establishment of monoclonal antibodies against the H and L subunits of the human ferritin and their application to the serum ferritin assay

人铁蛋白H、L亚基单克隆抗体的建立及其在血清铁蛋白检测中的应用

• 批准号: 61570614
• 负责人: KIMURA Shigeru
• 金额: $ 1.28万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570614/
• 关键词: Ferritin; H subunit; L subunit; Monoclonal antibodies; 酵素免疫測定法

Monoconal antibodies against human skeletal muscle ferritin were obtained by fusing mouse myeloma cell line (P3-X63-Ag8-U1)and mouse spleen lymphocytes two enzyme linked immunosorbent assays were established with those antibodies,one was specific to the H subunit and the other to the L subunit,In sera tested,levels of serum H subunit(sHsub) showed poor correlation with levels of serum ferritin in the anti-liver ferritin polyclonal assay(sFer-poly),but levels of serum L subunit(sLsub)had good correlation with levels of sFer-poly,sHsub levels were generally lower than sLsub levels. The positive and false positive rates of sHsub for cancer diagnosis were superior to those of sLsub in the thyroid tumors,but were comparable in other tumors.

Monoconal antibodies against human skeletal muscle ferritin were obtained by fusing mouse myeloma cell line (P3-X63-Ag8-U1)and mouse spleen lymphocytes two enzyme linked immunosorbent assays were established with those antibodies,one was specific to the H subunit and the other to the L subunit,In sera tested,levels of serum H亚基（SHSUB）与抗肝铁蛋白多克隆测定法（SFER-Poly）中血清铁蛋白水平的相关性较差，但是血清L亚基（SLSUB）水平与SFER-POLY水平良好相关，SHSUB水平通常低于SLSUB水平。 SHSUB的阳性和假阳性用于癌症诊断的速率优于甲状腺肿瘤中SLSUB的阳性和阳性率，但在其他肿瘤中也可比。