Organ-specificity of chum salmon Type I collagen

鲑鱼 I 型胶原蛋白的器官特异性

• 批准号: 02660210
• 负责人: KIMURA Shigeru
• 金额: $ 1.28万
• 依托单位: Tokyo University of Fisheries
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660210/
• 关键词: Chum salmon; Collagen; Subunit; Organ-specificity; シロザケ; サブユニット組成; 魚類

Wide distribution of the Type I collagen alpha3 chain in teleosts was shown by our previous studies. The alpha3 chain of salmonid fish was different in chromatographic behavior from those of other teleosts and could not be separated from the alpha2 chain by the conventional method using CM-cellulose at pH 4.8.The present study has demonstrated that the alpha3 chain of chum salmon can be isolated by CM-cellulose chromatography at pH 5.6 and is assumed to be the product of a third genetic locus which arose from the alpha1 gene. Furthermore, organ-specific occurrence of Type I collagen alpha3 chain was observed ; it was found in skin, swim bladder and bone but not in muscle.However, the structural significance of alpha3 chain in Type I collagen remains to be solved.

我们先前的研究表明，硬骨蛋白蛋白链的I型胶原蛋白alpha3链的广泛分布。鲑鱼鱼的α3链的色谱行为与其他硬骨蛋白的链不同，无法通过传统的方法与pH 4.8处的CM-纤维素相距于alpha2链，而本研究表明，Chum鲑鱼的alpha3链可以证明CM-Clulose Chimomatography可以通过pH 5.6和第三种遗传的产品分离出来。此外，观察到I型胶原蛋白α3链的器官特异性发生。它是在皮肤，游泳膀胱和骨骼中发现的，但在肌肉中没有发现。但是，α3链在I型胶原蛋白中的结构意义仍有待解决。

项目成果

Risako Matsui: "Characterization of an α3 chain from the skin Type I collagen of chum salmon (Oncorhynchus keta)" Comparative Biochemistry and Physiology. 99B. 171-174 (1991)

Risako Matsui：“鲑鱼（Oncorhynchus keta）皮肤 I 型胶原的 α3 链特征”比较生物化学和生理学 171-174（1991）。

Risako Matsui: "Characterization of an α3 chain from the skin Type I collagen of chum salmon(Oncorhynchus keta)" Comparative Biochemistry and Physiology. 99B. 171-174 (1991)

Risako Matsui：“鲑鱼（Oncorhynchus keta）皮肤 I 型胶原蛋白的 α3 链特征”比较生物化学和生理学 171-174（1991）。

Risako Matsui et al.: "Characterization of an alpha3 chain from the skin Type I collagen of chum salmon (Oncorhynchus keta)" Comp. Biochem. Physiol.99B. 171-174 (1991)

Risako Matsui 等人：“鲑鱼 (Oncorhynchus keta) 皮肤 I 型胶原蛋白的 α3 链的表征”Comp.

Risako MATSUI: "Characterization of an α3 chain from the skin Type I collagen of chum salmon (Oncorhynchus keta)" Comparative Biochemistry and Physiology. ?. (1991)

Risako MATSUI：“鲑鱼（Oncorhynchus keta）皮肤 I 型胶原蛋白的 α3 链特征”比较生物化学和生理学？（1991 年）。