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Kinetic analyses of allelopathic phenomena between microalage and environmental utilization of active substances

微藻与活性物质环境利用之间化感现象的动力学分析

基本信息

批准号：
62540498
负责人：
MOCHIDA Kazuo
金额：
$ 1.15万
依托单位：
Shimane University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

项目摘要

Allelopathic phenomena between microalage were examined using the dominant species of phytoplankton isolated from the brackish lake Nakanoumi.1. Interaction between dominant algae. The interactions between 2 species among 7 species of the algae were classified to 5 types. Especially, it was strongly suggested that the growth-inhibitory effect caused by Cymbella sp. was an allelopathic phenomenon which was attributable to the inhibitors secreted from the cells.2. Kinetic evaluation of interaction between algae. For analyzing kinetically the interactions between algae, two kinds of new type equations which were named the expanded logistic equation and the expanded Lotka-Volterra equation were derived. The former was used to express extensively the algal growth process during whole period including the decay or death phase. The latter was used to evaluate the degree of interaction between algae and thereby to quantify the degree as the interaction coefficients. Cymbella sp. was selected again as the most appropriate alga for pursuing the growth inhibitors.3. Separation and properties of growth inhibitors. Growth inhibitors produced by Cymbella sp. were found not only in the culture filtrate but also in the cell extracts. From the extract with 10 % acetone aqueous solution, 2 active fractions were separated by gel filtration using Bio-Gel P-2 and they were considered to be polar substances which the molecular weight was about 200. Their thermostability, ph-stability, and inhibitory effects on 4 species of the other algae were also confirmed. However, the structure identification and the environmental utilization of active chemicals were remained as further themes to be examined.

利用中之见咸水湖浮游植物优势种研究了微藻间的化感现象。优势藻类之间的相互作用。7种藻类中2种之间的相互作用可分为5种类型。特别是，这强烈表明桥弯藻的生长抑制作用是一种化感现象，这种化感作用是由细胞分泌的抑制剂引起的.藻类相互作用的动力学评价。为了从动力学角度分析藻类之间的相互作用，推导了两种新的方程，即扩展的Logistic方程和扩展的Lotka-Volterra方程。前者广泛地表达了藻类生长的整个过程，包括腐烂或死亡阶段。后者被用来评估藻类之间的相互作用的程度，从而量化的程度作为相互作用系数。再次选择桥弯藻为最适宜的生长因子.生长抑制剂的分离及性质研究。桥弯藻产生的生长抑制剂不仅存在于培养滤液中，也存在于细胞提取物中。从10%丙酮水溶液的提取物中，使用Bio-Gel P-2通过凝胶过滤分离出2个活性级分，认为它们是分子量约为200的极性物质。同时还证实了它们的热稳定性、pH稳定性以及对其它4种藻类的抑制作用。然而，活性化学品的结构鉴定和环境利用仍是有待进一步研究的主题。