Differential regulation at the wx locus of rice

水稻wx位点的差异调控

• 批准号: 62560010
• 负责人: SANO Yoshio
• 金额: $ 1.66万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62560010/
• 关键词: Gene expression; waxy gene; Rice; Amylose content; Multiple alleles; アミロース含量; アミロース

The waxy (wx) locus in rice, which specifies a major starchgranule-bound protein called Wx protein, is genetically well characterized. Our previous works revealed that in addition to wx, there are at least two nonwaxy alleles at the wx locus which regulate the amounts of the gene product as well as amylose in different ways. The present study was carried out to get more insights on to what extent the allelic differentiation at the wx locus is important for diversification in amylose content observed among nonwaxy rice cultivars. The results confirmed that among naturally occurring variants tested, amylose content is mainly determined by the allelic changes at the wx locus. In addition, the amount of the gene product was affected by effects of temperatures, modifiers and the gene dosage in a similar manner which the amylose content was affected. Those effects were expected to lead the amylose content to a polygenic trait in segregating populations as often reported in rice, even though the amylose content was simply controlled by the amount of Wx protein. The allelic differentiation at the wx locus was also discussed in relation to the evolutionary significances of the gene regulation.

水稻中的蜡质基因（wx）是一种主要的淀粉粒结合蛋白，被称为Wx蛋白。我们以前的工作表明，除了wx，至少有两个nonwaxy等位基因在wx基因座，调节基因产物的数量，以及直链淀粉以不同的方式。本研究的目的是进一步了解wx基因座的等位基因分化在多大程度上对非糯水稻品种直链淀粉含量的多样性具有重要意义。结果证实，在测试的天然存在的变体中，直链淀粉含量主要由wx基因座处的等位基因变化决定。此外，温度、修饰剂和基因剂量对基因产物量的影响与对直链淀粉含量的影响相似。这些影响预计将导致分离群体中的直链淀粉含量的多基因性状在水稻中经常报道，即使直链淀粉含量是简单地由Wx蛋白的量控制。本文还讨论了wx位点的等位基因分化与基因调控的进化意义。

项目成果

Nishimura, M. and Y. Sano: "Intermediate amylose content determined by an allele at the wx locus." Rice Genet. Newslett. 6: 95-96, 1989.

Nishimura, M. 和 Y. Sano：“中间直链淀粉含量由 wx 基因座的等位基因决定。”

M.Nishimura & Y.Sano: "Intermediate amylose content determined by an allele at the wx locus." Rice Genetics Newsletter. 6. 95-96 (1989)

西村先生

Y.Sano: "Oryza glaberrima and O.sativa carry wx,Se-1,A and Rc at the same chromosomal locations." Rice Genetics Newsletter. 5. 66-67 (1988)

Y.Sano：“Oryza glaberrima 和 O. sativa 在相同的染色体位置携带 wx、Se-1、A 和 Rc。”

Y.Sano: "The genic nature of gamete eliminator in rice." Genetics. (1990)

Y.Sano：“水稻配子消除剂的基因性质。”

Sano, Y.: "The direction of pollen flow between two co-occurring rice species, Oryza sativa and O. glaberrima" Heredity 63: 353-357, 1989.

Sano, Y.：“两种共生水稻品种 Oryza sativa 和 O. glaberrima 之间的花粉流动方向”，Heredity 63：353-357，1989。