Morphological and biochemical studies on the development and function of paraneurons in the branchiogenous organs

鳃生器官旁神经元发育和功能的形态学和生化研究

• 批准号: 62560288
• 负责人: TANIGUCHI Kazuyuki
• 金额: $ 1.22万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62560288/
• 关键词: Branchiogenous organs; Thyroid gland; Parathyroid gland; Ultimobranchial body; Paraneurons; Calcium metabolism; Image - processing system; Immunohistochemistry; カルシトニン分泌細胞; パラソルモン分泌細胞; C細胞; 画像解析; 免疫細胞化学

The thyroid gland, Parathyroid gland, thymus, Palatine tonsil and ultimobranchial body are collectively called branchiogenous organs. Among them, the thyroid and parathyroid glands contain C cells and chief cells, respectively. These cells are specific endocrine cells, called paraneurons, and secrete calcitonin(CT) and parathormone(PTH), respectively, to be concerned with the calcium(Ca) metabolism. In the present study, the ontogeny of paraneurons in the branchiogendus organs, the influence of vitamin D(VD) administration or Ca and VD deficiency on the Ca metabolism during gestation, the influence of vitamin A(VA) deficiency on the pituitary-thyroid and pituitary- gonadal systems, and morphological changes of the pituitary endocrine cells in the aging, gestation and hibernation are examined by immunobistochemistry, morphometry and radioimmunoassay to reveal morphologically and biochemically the development and function of parangurans in the branchiogenous organs. As the results, the morphometric analysis with image-processing system revealed statistically the remarkable increase of the volume of the parathyroid gland in the Ca- and VD- deficient rats, the significant increase of the area of TSH cells of the pituitary gland in the VA- deficient rats, and the significant decrease of the area of TSH cells in the hibernating Japanese long-fingered bats. The embryological study on the development of branchiogenous organs and their paraneurons revealed by immunohistochemistry the appearance of the primordia of the thyroid gland and ultimobranchial body on day 9 of gestation, the appearance of the primordium of the parathyroid glared on day 10.25 of gestation, the fusion of the ultimobranchial body with the thyroid gland on day 12 of gestation, and the appearance of CT-immunoreactive cells in the region of the thyroid gland derived from the ultimobranchial body.

甲状腺、甲状旁腺、胸腺、腭扁桃体和鳃裂体统称为鳃裂器官。其中，甲状腺和甲状旁腺分别含有C细胞和主细胞。这些细胞是特殊的内分泌细胞，称为副神经元，分别分泌降钙素（CT）和甲状旁激素（PTH），与钙（Ca）代谢有关。本研究采用免疫组化的方法研究了副神经元在鳃部器官的发生，维生素D（VD）或Ca和VD缺乏对妊娠期Ca代谢的影响，维生素A（VA）缺乏对垂体-甲状腺和垂体-性腺系统的影响，以及衰老、妊娠和冬眠期间垂体内分泌细胞的形态学变化。形态测定学和放射免疫分析法揭示鳃源性器官的形态和生化发育和功能。结果显示，Ca-和VD-缺乏症大鼠的甲状旁腺体积显著增加，VA-缺乏症大鼠垂体TSH细胞面积显著增加，冬眠日本长指蝙蝠的TSH细胞面积显著减少。免疫组化法观察了妊娠第9天甲状腺原基和甲状旁腺原基的形态，妊娠第10.25天甲状旁腺原基的形态，妊娠第12天甲状旁腺原基与甲状腺的融合，妊娠第12天甲状旁腺原基与甲状腺的融合。以及来自鳃裂小体的甲状腺区域的ct免疫反应细胞的出现。

项目成果

Mikami,Shinichi: "Immunocytochemical studies on the pituitary pars distalis of the Japanese long-fingered bat,Miniopterus schreibersii fuliginosus." Cell Tissue Res.251. 291-299 (1988)

Mikami、Shinichi：“日本长指蝙蝠 Miniopterus schreibersii fuliginosus 垂体远端的免疫细胞化学研究。”

Taniguchi,Kazuyuki: "Immunocytochemical and morphomtric studies on the pars distalis of the golden hamster from perinatal to senile stage." Jpn.J.Vet.Sci.51. 893-903 (1989)

Taniguchi，Kazuyuki：“金仓鼠从围产期到老年期远端部的免疫细胞化学和形态测量研究。”

Naito, Yoshihisa: Jpn. J. Vet. Sci.49. 477-483 (1987)

内藤义久：日本。

Mikami,Shinichi: "Immunocytochemical localization of neuropeptides in the hypothalamus of Japanese long-fingered bat,Miniopterus schreibersii fuliginosus." Cell Tissue Res.254. 49-57 (1988)

Mikami，Shinichi：“日本长指蝙蝠 Miniopterus schreibersii fuliginosus 下丘脑中神经肽的免疫细胞化学定位。”

Taniguchi, Kazuyuki: "Immunocytochemical and morphometric studies on the pars distalis of the golden hamster from perinatal to senile stage." Jpn. J. Vet. Sci., 51, 893-903, 1989.

Taniguchi、Kazuyuki：“对金仓鼠从围产期到老年期远端部的免疫细胞化学和形态测量研究。”