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Analysis of olfactory information transmission mechanism by bio-imaging

利用生物影像分析嗅觉信息传递机制

基本信息

批准号：
11660293
负责人：
TANIGUCHI Kazuyuki
金额：
$ 2.3万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660293/
关键词：
olfactory epithelium vomeronasal organ septal olfactory organ of Masera middle chamber epithelium sugar residues lectin histochemistry immunohistochemistry bio-imaging 嗅覚系主嗅球副嗅球レクチン嗅糸球

项目摘要

In the present study, various examinations were carried out to clarify the details of olfactory information transmision mechanism in the vertebrates. In Xenopus laevis, the main and lower chambers were lined by the olfactory epithelium and vomer onasal organ, respectively, and the middle chamber was lined by a specific sensory epithelium, the middle chamber epithelium. These three olfactory receptor organs showed the different expression patterns of glycoconjugates with each other. Judging from the absence of associated olfactory gland and the difference in fine structure of receptor cells, the middle chamber epithelium was suggested to be an olfactory organ to detect odoriferous molecules in water. In addition, the olfactory epithelium, middle chamber epithelium, vomeronasal organ projected to the dorsal region of the main olfactory bulb (MOB), ventral region of the MOB, and the accessory olfactory bulb, respectively. These findings suggested the presence of three independent olfactory pathways in Xenopus laevis. Then, olfactory glomeruli of the hamster MOB showed the differential expression patterns of glycoconjugates with 13 kinds of lectins. These results suggested that individual glomeruli were different with each other in the kind and volume of sugar residues from the viewpoint of probability. Lastly, the three olfactory receptor organs of rats, i.e., olfactory epithelium, vomer onasal organ, and septal olfactory organ of Masera (MO), developed in common from the primitive olfactory epithelium lining the embryonic nasal pit. In the case of the MO, the primitive olfactory epithelium lining the nasal septum transformed to the respiratory epithelium around the MO, and the remaining primitive olfactory epithelium differentiated to the MO on the nasal septum. Further, results by bio-imaging were not satisfactory in the present study.

本研究对脊椎动物的嗅觉信息传递机制进行了多方面的研究。在非洲爪哇，主腔和下腔分别由嗅觉上皮和鼻腔器官构成，中腔由一种特殊的感觉上皮--中腔上皮构成。这三种嗅觉感受器呈现出不同的糖结合物表达模式。从没有伴生嗅腺和受体细胞精细结构的差异来看，中室上皮可能是探测水中气味分子的嗅觉器官。此外，嗅觉上皮、中室上皮和犁鼻器分别投射到主嗅球背侧区、副嗅球腹侧区和副嗅球。这些发现表明非洲爪哇存在三条独立的嗅觉通路。然后，地鼠嗅球与13种凝集素的糖偶联物呈现出不同的表达模式。这些结果表明，从概率的角度来看，不同的肾小球在糖残留的种类和体积上是不同的。最后，大鼠的三种嗅觉感受器，即嗅觉上皮、鼻蜗器和Masera隔嗅器(MO)，都是由排列在胚胎鼻窝内的原始嗅觉上皮共同发育而来的。在MO的情况下，鼻中隔周围的原始嗅觉上皮向MO周围的呼吸上皮转化，剩余的原始嗅觉上皮分化为鼻中隔的MO。此外，生物成像的结果在本研究中并不令人满意。