Epidemiological study on Lymphocytic choriomeningitis virus in wild rodents of Japan

1985 ~ 1989年在7个码头捕获的129只小鼠中，LCM抗原阳性9只（7.0%）。1985年31只小鼠中有6只（19.4%）阳性，1986年23只小鼠中有3只（12.5%）阳性。1988年和1989年捕获的74只小鼠均为阴性。1985年和1986年在乌山桥-新高码头捕获的17只小鼠中有7只（42.1%）呈阳性，在本目码头捕获的23只小鼠中有2只（8.7%）呈阳性，其他码头捕获的小鼠均呈阴性。9只阳性小鼠中5只在同一仓库同时捕获。横滨港LCM病毒流行区明显集中在某一区域。LCM在该地区小鼠的流行时间不长。在欧洲农村地区，LCM病毒在家鼠中长期存在地方性疫源地。虽然目前对仓储型家鼠的生态学研究尚不清楚，但可以推测，仓储型家鼠的种群数量依赖于仓储型家鼠的种类。1985年捕获5只阳性小鼠的t…More仓库没有获得足够的样本，可能是由于仓库库存的变化。在1988-1989年，我们从仓库中只获得了一个样品，这是阴性的。我们没能追踪到从仓库分散到其他地方的阳性老鼠。由于横滨港是日本最大的国际港口之一，因此有可能认为LCM病毒会通过船只从外国侵入日本。虽然小asawara群岛Chichi-jima岛上的小鼠是通过对其细胞的DNA分析发现的，它们属于家养m.s musculus （Suzuki和Morita：未发表的数据），但1989年在岛上捕获的82只小鼠的血清对LCM病毒均呈阴性（Morita和Yabe未发表的数据）。我们还调查了千叶县和静冈县家养鼠（A. speciosus， A. argenteus）中LCM病毒的流行情况。千叶县159株和静冈县288株中分别有1株和1株的IFA检测呈阳性，但Western blotting分析结果未得到证实。静冈县49只阿根廷伊蚊无阳性。结果在横滨港家鼠中发现LCM病毒的存在。有必要进行更深入的研究，以澄清该病毒在日本的流行情况。少

Nine out of 129 mice captured from 7 piers from 1985 to 1989 were positive against LCM antigen(7.0%). Six out of 31 mice (19.4%) and 3 out of 23 mice (12.5%) were positive in 1985 and 19 86 respectively. All 74 mice captured in 1988 and 1989 were negative. Although 7 out of 17 mice (42.1%) in Oosanbashi-Shinkoh pier and 2 out of 23 (8.7%) in Honmoku pier were positive in 1985 and 1986, All mice captured in other piers were negative. Five out of 9 positive mice were captured in the same warehouse at same time. Endemic area of LCM virus in Yokohama port was clearly focused into the certain area. Endemicity of LCM in mice in the area was not prolonged for long time. In Europe, endemic focus of LCM virus in house mice was maintained for long period in a rural area. Although, ecology of house mouse in warehouses is not clearly investigated, it can be speculated that population of house mouse is depend on sorts of stocks in warehouses. The reason why we did not obtained enough samples from t … More he warehouse where we captured 5 positive mice in 1985, may be due to changes of stocks in the warehouse. In 1988-1989, we obtained from the warehouse only one sample , which is negative. We failed to trace positive mice scattered from the warehouse to other places.It may be thought that LCM virus would invade into Japan from foreign countries through ships because Yokohama port is one of the big international ports in Japan. Although the mice in Chichi-jima island in Ogasawara islands are revealed by using DNA analysis of their cells as genetically foreign origin and belong to M. musculus domesticus (Suzuki and Morita: unpublished data), all sera of 82 mice captured in the island in 1989 were negative against LCM virus (Morita and Yabe unpublished data).We also investigated prevalence of LCM virus in domestic Apodemus mice ( A. speciosus, A. argenteus) from Chiba prefecture and Shizuoka prefecture.In A. speciosus, 1 out of 159 from Chiba prefecture and 1 out of 288 from Shizuoka prefecture were positive in IFA test however, the result was not confirmed by Western blotting analysis. None of 49 A. argenteus from Shizuoka prefecture was positive. Consequently existence of LCM virus was revealed in house mice in Yokoham a port. More intensive studies are necessary to clarify prevalence of the virus in Japan. Less