Epidemiological study on Lymphocytic choriomeningitis virus in wild rodents of Japan

从1985 ~ 1989年7个码头捕获的129只小鼠中，有9只小鼠抗LCM抗原阳性，阳性率为7.0%。1985年和1986年分别在31只和23只小鼠中检出6只（19.4%）和3只（12.5%）。1988年和1989年捕获的74只小鼠均为阴性。1985年和1986年在大山桥-新光码头捕获的17只小鼠中有7只（42.1%）和在本木码头捕获的23只小鼠中有2只（8.7%）为阳性，但在其他码头捕获的小鼠均为阴性。9只阳性鼠中有5只在同一仓库同时捕获。LCM病毒在横滨港的流行区明显集中在一定的区域内。LCM在该地区小鼠中的流行时间不长。在欧洲，LCM病毒在家鼠中的地方性疫源地在农村地区维持了很长一段时间。虽然目前对仓库中家鼠的生态学研究还不清楚，但可以推测，仓库中家鼠的种群数量取决于仓库中的存货种类。我们之所以没有从t中获得足够的样本， 关于我们 1985年我们捕获5只阳性小鼠的仓库，可能是由于仓库库存变化所致。1988-1989年，我们只从仓库获得一个样本，结果是阴性。我们未能追踪到从仓库散落到其他地方的阳性小鼠，可能认为LCM病毒会通过船只从外国入侵日本，因为横滨港是日本的国际大港口之一。虽然小笠原群岛秩次岛的小鼠经细胞DNA分析显示为遗传学上的外来种，属于M。1989年在日本岛上捕获的82只小鼠血清均为LCM病毒阴性（Morita和Yabe未发表的资料）。speciosus，A. argenteus）。千叶县159株和静冈县288株中各有1株IFA阳性，但未得到Western blotting的证实。没有49 A。来自静冈县的银汉鱼为阳性。因此，在横滨港的家鼠中发现了LCM病毒的存在。有必要进行更深入的研究，以澄清该病毒在日本的流行情况。少

Nine out of 129 mice captured from 7 piers from 1985 to 1989 were positive against LCM antigen(7.0%). Six out of 31 mice (19.4%) and 3 out of 23 mice (12.5%) were positive in 1985 and 19 86 respectively. All 74 mice captured in 1988 and 1989 were negative. Although 7 out of 17 mice (42.1%) in Oosanbashi-Shinkoh pier and 2 out of 23 (8.7%) in Honmoku pier were positive in 1985 and 1986, All mice captured in other piers were negative. Five out of 9 positive mice were captured in the same warehouse at same time. Endemic area of LCM virus in Yokohama port was clearly focused into the certain area. Endemicity of LCM in mice in the area was not prolonged for long time. In Europe, endemic focus of LCM virus in house mice was maintained for long period in a rural area. Although, ecology of house mouse in warehouses is not clearly investigated, it can be speculated that population of house mouse is depend on sorts of stocks in warehouses. The reason why we did not obtained enough samples from t … More he warehouse where we captured 5 positive mice in 1985, may be due to changes of stocks in the warehouse. In 1988-1989, we obtained from the warehouse only one sample , which is negative. We failed to trace positive mice scattered from the warehouse to other places.It may be thought that LCM virus would invade into Japan from foreign countries through ships because Yokohama port is one of the big international ports in Japan. Although the mice in Chichi-jima island in Ogasawara islands are revealed by using DNA analysis of their cells as genetically foreign origin and belong to M. musculus domesticus (Suzuki and Morita: unpublished data), all sera of 82 mice captured in the island in 1989 were negative against LCM virus (Morita and Yabe unpublished data).We also investigated prevalence of LCM virus in domestic Apodemus mice ( A. speciosus, A. argenteus) from Chiba prefecture and Shizuoka prefecture.In A. speciosus, 1 out of 159 from Chiba prefecture and 1 out of 288 from Shizuoka prefecture were positive in IFA test however, the result was not confirmed by Western blotting analysis. None of 49 A. argenteus from Shizuoka prefecture was positive. Consequently existence of LCM virus was revealed in house mice in Yokoham a port. More intensive studies are necessary to clarify prevalence of the virus in Japan. Less