权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

ISOLATION AND CHARACTERIZATION OF SPOTTED FEVER GROUP RICKETTSIA IN NONENDEMIC AREA OF JAPANESE SPOTTED FEVER

日本斑疹热非流行区斑疹热群立克次体的分离与鉴定

基本信息

批准号：
07660425
负责人：
MORITA Chiharu
金额：
$ 1.09万
依托单位：
RAKUNO-GAKUEN UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

In 1995FY,wild mice were collected from the conserved forest in Nopporo, Ebetsu-shi and investigated prevalence of the antibody against spotted fever group rickettsia (SFGR). High prevalence of the antibody was correlated to infestation of ticks. In 1996FY wild mice and ticks were also collected from Towada-shi, Aomori prefecture. High prevalence and titer of the antibody in wild mice were found in the area as well as Nopporo.Ebetsu-shi.Attempts to isolate SFGR from wild mice and ticks collected from the both areas were performed by using mice inoculation and direct inoculation to L cells or Vero cells by centrifugation method. It has not succeeded in isolation of SFGR or SFGR like organisms. The attempts is continued still now. In addition, the existence of SFGR was not revealed in original samples from wild mice by PCR using common primers of SFGR.The result will be interpreted that evidence of the prevalence of the antibody in wild mice is complicated problems.Since the reports on SFGR is not so common in Thailand, the relationship between SFGR in Thailand and Japanese spotted fever rickettsia was investigated using wild rat sera collected in the 1970s. It was found that Bandicota indica was one of the important reservoirs of SFGR in Thailand and TT-118, pathogenisity for human of which was not clear, or antigenically related organisms was high prevalent but Japanese spotted fever SFGR was not so common in Thailand.SFGR in southern Africa except Rep.South Africa and Zimbabwe was not well investigated. We obtained about 300 human sera from 3 areas in Zambia. High prevalence of the antibody against SFGR (R.conorii) was found in 2 of the 3 areas where were cattle production center in Zambia.

1995财年，从江别市野幌的保护森林中采集了野生小鼠，并调查了针对斑疹热群立克次体（SFGR）的抗体的流行情况。该抗体的高流行率与蜱虫的感染有关。 1996财年，还从青森县十和田市采集了野生小鼠和蜱虫。该地区以及江别市野幌市的野生小鼠中均发现了该抗体的高流行率和滴度。通过使用小鼠接种以及通过离心法直接接种L细胞或Vero细胞，尝试从这两个地区收集的野生小鼠和蜱中分离SFGR。它尚未成功分离 SFGR 或 SFGR 类生物体。这些尝试现在仍在继续。此外，使用SFGR通用引物进行PCR，在野生小鼠原始样本中并未发现SFGR的存在。结果将解释为野生小鼠中抗体流行的证据是复杂的问题。由于SFGR的报道在泰国并不常见，因此使用20世纪70年代收集的野生大鼠血清研究了泰国SFGR与日本斑疹热立克次体之间的关系。研究发现Bandicota indica是泰国SFGR的重要宿主之一，而TT-118对人类的致病性尚不清楚，或抗原相关生物广泛流行，但日本斑疹热SFGR在泰国并不常见。除南非和津巴布韦外，南部非洲的SFGR尚未得到充分调查。我们从赞比亚的3个地区获得了约300份人类血清。在赞比亚的 3 个养牛中心地区中，有 2 个地区发现了针对 SFGR（R.conorii）的抗体的高流行。