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Studies for Abnormal Expression of Interleukin-2 Receptor in Patients with Systemic Lupus Erythematosus

系统性红斑狼疮患者白细胞介素2受体异常表达的研究

基本信息

批准号：
62570285
负责人：
KUMAGAI Shunichi
金额：
$ 1.28万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570285/
关键词：
Systemic Lupus Erythematosus Interleukin Interleukin-2 Interleukin-2 receptor 強皮症 IL-2 IL-2レセプター T細胞

项目摘要

The expression of interleukin-2 receptor (IL-2R) in patients with systemic lupus erythematosus (SLE) was investigated in order to clarify the causes of impaired functions of their T cells.1. Studies for IL-2 production of peripheral lymphocytes.The IL-2 production of PHA-stimulated lymphocytes was significantly decreased in patients with SLE. However, PSS patients showed markedly enhanced IL-2 production, which was derived from hyperactivity of thier CD4-positive T cells.2. Studies for expression of IL-2R on PHA-activated peripheral lymphocytes in patients with SLE.(1) When the expression of IL-2R/p55 was examined using FITC-conjugated or ^<125>-I labeled anti-Tac monoclonal antibody, both the percentage of Tac-positive T cells and the number of Tac epitope were not different between SLE patients and normal subjects.(2) The IL-2R expression of PHA-activated lymphocytes from SLE patients was further studied by the scatchard plot analysis using ^<125>-labeled recombinant IL-2. The prominent defect in expression of high-affinity IL-2R was demonstrated in patients with SLE.3. Using FITC-conjugated IL-2, we have established the new and easy method which mainly detected the high-affinity IL-2R. By use of this mithod, the defective expression of high affinty IL-2R was clearly shown on activated t cells of SLE patients in spite of thier normal expression of Tac antigen. The defect was more prominent on thier CD4-positive T cells.In summary, T cells of SLE patients was shown to have a defect in expression or regulation of high-affinity IL-2R, which may cause various dysfunctions in the patients.

本研究通过检测系统性红斑狼疮（SLE）患者外周血T细胞白细胞介素2受体（IL-2 R）的表达，探讨SLE患者T细胞功能受损的原因.外周血淋巴细胞产生IL-2的研究SLE患者经PHA刺激的淋巴细胞产生IL-2的能力明显下降。然而，PSS患者的IL-2产生明显增加，这是由于其CD 4阳性T细胞的过度活跃. SLE患者PHA激活的外周血淋巴细胞IL-2 R表达的研究(1)用FITC标记的抗Tac单克隆抗体或~（131）I标记的抗Tac单克隆抗体检测IL-2 R/p55的表达<125>，结果表明SLE患者和正常人的Tac阳性T细胞百分率和Tac表位数均无差异。(2)使用β-标记的重组IL-2通过Scatchard图分析进一步研究了来自SLE患者的PHA活化淋巴细胞的IL-2 R表达<125>。SLE患者高亲和力IL-2 R的表达存在明显缺陷.利用FITC标记的IL-2，建立了一种新的、简便的方法，主要用于检测高亲和力的IL-2 R。结果表明，SLE患者活化的T细胞表面Tac抗原表达正常，但高亲和力IL-2 R表达缺失。SLE患者的T细胞在表达或调节高亲和力IL-2 R方面存在缺陷，这可能导致SLE患者的多种功能障碍。