Development of a new assay system for transcription factor activity using FCS

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First, we tried to develop a new method to measure transcription activity in clinical samples. We did so using FCS technology and ELISA technique, which showed a good correlation with each other. Second, We measured transcriptional activity in PBMC of rheumatoid arthritis patients (RA) and found that NFkB show variable titers in RA patients. In four patients receiving biologics treatment, NFkB was considered best as a marker for disease activity among NFkB, STAT3, AP-1, and NFAT. Then we checked NFkB family activity and found p50 and p65 were elevated in RA patients. Finally, we showed that p65 activity correlates with RA activity in RA patients receiving biologics therapy. Further experiments will be needed to determine whether this technique is useful to predict the efficacy of biologic therapies.