Chromosomal analysis of woodchuck hepatocellular carcinoma utilizing in situ hybridization technique

点击翻译按钮获取中文摘要

The chromosomal sites of WHV DNA integration were identified in WH257 GE10 by an in situ hybridization technique. ^3H-labelled WHC DNA was assigned to the G-banded diagram which was determined for the first time in the woodchuck chromosomes. WHV DNA was integrated into two sites; 33 region of the long arm of chromosome 6 (6q33) and 31 region of the long arm of chromosome 8 (8q31). Chromosomal sites of WHV DNA integration were stable during the passage periods analyzed. These findings suggest that WHV DNA integration into the chromosomes of WH257 GE10 might be closely associated with the pathogenesis of a woodchuck hepatocellular carcinoma, and that integrated WHV DNA might play some role in the maintenance of the characteristics as cancer cells.