Genomics of liver cells anォ liver cancer cells

肝细胞和肝癌细胞的基因组学

基本信息

  • 批准号:
    11307010
  • 负责人:
  • 金额:
    $ 19.34万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 财政年份:
    1999
  • 资助国家:
    日本
  • 起止时间:
    1999 至 2001
  • 项目状态:
    已结题

项目摘要

Gene expression profile of normal liver, liver tissue from chronic hepatitis C (CH-C) and liver cancer (HCC) were analyzed by SAGE (serial analysis of gene expression) method. Total of 30,982 sequence tags were separated from normal tissue, in which 8,596 tags were derived from unique genes. The genes highly expressed in the normal liver were those encoding plasma proteins (>21.8% of total transcripts), cytoplasmic proteins (>8.6%), enzymes (>4.8%), protease inhibitors (>1.7%), complements (>1.1!%), and coagulation factors (>0.75%). About 13.9% of all transcripts encoded genes not reported in GenBank thus far. Tags from the HCC library exhibited the existence of many differentially expressed genes compared with those from the CH-C and NL libraries. Up-regulation of IFN-gamma inducible genes and oxidative stress-inducible genes were identified in both the CH-C and HCC libraries, and some unpublished new genes were specifically up- or down-regulated in the HCC library.DNA micro array containing 1,080 genes covering various categories of genes including oncogenes, tumor suppressor genes, apoptosis related genes, cell cycle related genes were originally developed. Hierarchical clustering analysis of the gene expression profiles showed that Hepatitis B and C virus infection, but not age, sex, or histology of hepatitis, were significant factors determining clustering (P 0.05). In hepatitis B tissue lesions, genes involved in inflammation were predominant, whereas in hepatitis C, expression of anti-inflammatory response genes was relatively dominant. In addition, clustering analysis of DNA micro array data showed that tumor marker alpha-fetoprotein producing hepatoma cell lines shares common expression profile of genes in various categories. Analysis of gene expression profile of HCC tissues showed that some genes had characteristic gene expression pattern according to tumor differentiation.
应用SAGE(serialanalysisofgene expression)方法分析了正常肝、慢性丙型肝炎(CH-C)肝组织和肝癌(HCC)组织的基因表达谱。从正常组织中共分离出30,982个序列标签,其中8,596个标签来自独特基因。在正常肝脏中高度表达的基因是那些编码血浆蛋白(>21.8%的总转录物)、胞质蛋白(>8.6%)、酶(>4.8%)、蛋白酶抑制剂(>1.7%)、补体(> 1.1%)、凝血因子(>0.75%)。约13.9%的转录本编码的基因尚未在GenBank中报道。与CH-C和NL文库相比,HCC文库中的标签显示出许多差异表达基因的存在。在CH-C文库和HCC文库中均发现了IFN-γ诱导基因和氧化应激诱导基因的上调表达,并发现了一些未发表的新基因在HCC文库中特异性上调或下调表达。基因表达谱的层次聚类分析显示,B型和C型肝炎病毒感染,而不是年龄,性别或肝炎组织学,是决定聚类的重要因素(P 0.05)。在B型肝炎组织病变中,参与炎症反应的基因占优势,而在丙型肝炎中,抗炎反应基因的表达相对占优势。此外,DNA微阵列数据的聚类分析表明,肿瘤标志物甲胎蛋白产生肝癌细胞系共享不同类别的基因的共同表达谱。肝癌组织基因表达谱分析表明,某些基因具有与肿瘤分化程度相关的特征性基因表达谱。

项目成果

期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Taro Yamashita: "Comprehensive gene expression profile of a normal human liver"Biochemical and Biophysical Research Communications. (in press).
山下太郎:“正常人类肝脏的综合基因表达谱”生物化学和生物物理研究通讯。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Katsuro Koike: "Survey of hepatitis B virus co-infection in hepatitis C virus infected patients suffering from chronic hepatitis and hepatocellular carcinoma in Japan"Japanese Journal of Cancer Research. 90. 1270-1272 (1999)
小池胜郎:“日本慢性肝炎和肝细胞癌丙型肝炎病毒感染者中乙型肝炎病毒合并感染的调查”日本癌症研究杂志。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Shirota Y et al.: "Identification of differential expressed genes in hepatocellular carcinoma with cDNA microarrays"Hepatology. 33・4. 832-840 (2001)
Shirota Y等:“利用cDNA微阵列鉴定肝细胞癌中的差异表达基因”Hepatology 832-840(2001)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Shirota Y et al.: "Identification of differential expressed genes in hepatocellular carcinoma with cDNA micoarrys"Hepatology. 33・4. 832-840 (2001)
Shirota Y等:“用cDNA微阵列鉴定肝细胞癌中的差异表达基因”Hepatology 832-840(2001)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Shirora,Y.,Kaneko,S.,Honda,M.,Kawai,H.F.,Kobayashi,K.: "Identification of differentially expressed genes in hepatocellular carcinoma with cDNA microarrays"Hepatology in print. 33・4. (2001)
Shirora, Y.、Kaneko, S.、Honda, M.、Kawai, H.F.、Kobayashi, K.:“利用 cDNA 微阵列鉴定肝细胞癌中的差异表达基因”,《肝病学》印刷版 (2001)。
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    0
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KOBAYASHI Kenichi其他文献

豪雨災害の現状と技術開発
暴雨灾害现状及技术发展
  • DOI:
  • 发表时间:
    2018
  • 期刊:
  • 影响因子:
    0
  • 作者:
    HAYASHI Motoya;KOBAYASHI Kenichi;KIM Hoon;KAIHARA Noriko;二瓶泰雄
  • 通讯作者:
    二瓶泰雄
The state of the indoor air environment in buildings and related tasks in Japan〈Review〉
日本建筑物室内空气环境状况及相关工作〈回顾〉

KOBAYASHI Kenichi的其他文献

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{{ truncateString('KOBAYASHI Kenichi', 18)}}的其他基金

Lead discovery for Alzheimer's disease through synthetic organic chemistry
通过有机合成化学引领阿尔茨海默病的发现
  • 批准号:
    20K06951
  • 财政年份:
    2020
  • 资助金额:
    $ 19.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Study on the Functions and Facility Planning of Community-based integrated Hospital Wards
社区综合医院病房功能及设施规划研究
  • 批准号:
    15K06392
  • 财政年份:
    2015
  • 资助金额:
    $ 19.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Studies on the regulation of the reabsorption of nutrition by vitamin B-12 in the Kidney
维生素B-12对肾脏营养重吸收调节的研究
  • 批准号:
    23780144
  • 财政年份:
    2011
  • 资助金额:
    $ 19.34万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Research of the settlements of the Jomon period by radiocarbon dating
绳文时代聚落的放射性碳测年研究
  • 批准号:
    22520774
  • 财政年份:
    2010
  • 资助金额:
    $ 19.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A study on guideline for emergency medical center considering regionalism and management style
考虑区域性和管理方式的急诊医疗中心指南研究
  • 批准号:
    22560636
  • 财政年份:
    2010
  • 资助金额:
    $ 19.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
High-Temperature Creep Testing Method using Small Sample Specimens
使用小样本的高温蠕变测试方法
  • 批准号:
    22560072
  • 财政年份:
    2010
  • 资助金额:
    $ 19.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Measuring System for Bone Mineral Content by Panoramic Radiography
全景X线骨矿物质含量测量系统
  • 批准号:
    11557148
  • 财政年份:
    1999
  • 资助金额:
    $ 19.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
A Study on the Ancient Weapons -An Experimental Consideration on the Practical Use-
古代兵器研究-实用化的实验思考-
  • 批准号:
    09610413
  • 财政年份:
    1997
  • 资助金额:
    $ 19.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A Comparative Study on the Ancient Funeral in Korea and Japan
韩国和日本古代丧葬的比较研究
  • 批准号:
    09044017
  • 财政年份:
    1997
  • 资助金额:
    $ 19.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A).
Basic study of gene therapy for hepatocellular carcinoma
肝细胞癌基因治疗的基础研究
  • 批准号:
    07457133
  • 财政年份:
    1995
  • 资助金额:
    $ 19.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

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联合基因组重测序和10× Genomics scRNA-Seq解析乌骨鸡胸肌黑色素转运的分子机制
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REU 网站:基因组学和神经科学本科生的研究经验
  • 批准号:
    2349224
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    2024
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    $ 19.34万
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    2349410
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Director of Functional Genomics Initiative
功能基因组学计划主任
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    MR/Z000068/1
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会议:扩大第21届国际水稻功能基因组学研讨会(ISRFG)的参与范围
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