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Ultracytochemical characteristics of blasts in abnormal myelopoiesis of Down's syndrome

唐氏综合征骨髓增生异常原始细胞的超细胞化学特征

基本信息

批准号：
62570437
负责人：
EGUCHI Mitsuoki
金额：
$ 0.77万
依托单位：
Dokkyo University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

Ultrastructural and ultracytochemical sturies were performed on blasts from 15 Down's syndrome neonates with transient myeloproliferative disorder (TMD). And 12 of them with precise immunological and clinical data were compared with 13 Down's syndrome patients with megakaryoblastic leukemia (MKL) in order to clarify the cytological nature of the blast cells. Average platelet peroxidase-positivity in blast cells of TMD were similar to those of MKL. Blast cells from TMD contained alpha granules, myeloperoxidase-positive granules, electron lucent granules, granules with membrane components, basophil-like granules and granules with ferritin particles. Blast cells from MKL with down's syndrome contained electron lucent granules, granules with membrane components and a few basophil granules, but lacked alpha granules, MPO-positive granules and granules with ferritin particles. A demarcation membrane system was observed in blasts from TMD, but not in MKL with Down's syndrome.These findings suggest that blast cells in TMD show differentiation to megakaryocytes, neutrophils, basophils, and erythroblasts, while blast cells from MKL show limited differentiation to immature megakaryocytes, erythroblasts and a few basophils. And these results coinside with culture studies which showed differentiation of TMD blasts to various types of blood cells.

对 15 名患有短暂性骨髓增生性疾病 (TMD) 的唐氏综合症新生儿的原始细胞进行了超微结构和超细胞化学检查。并将其中12个具有精确免疫学和临床数据的细胞与13个患有巨核细胞白血病（MKL）的唐氏综合症患者进行比较，以阐明母细胞的细胞学性质。 TMD 母细胞中的平均血小板过氧化物酶阳性率与 MKL 相似。来自TMD的母细胞含有α颗粒、髓过氧化物酶阳性颗粒、电子透明颗粒、具有膜成分的颗粒、嗜碱性粒细胞样颗粒和具有铁蛋白颗粒的颗粒。来自患有唐氏综合症的MKL的母细胞含有电子透明颗粒、具有膜成分的颗粒和少量嗜碱细胞颗粒，但缺乏α颗粒、MPO阳性颗粒和具有铁蛋白颗粒的颗粒。在TMD的原始细胞中观察到了分界膜系统，但在患有唐氏综合症的MKL中却没有观察到。这些发现表明，TMD中的原始细胞显示出向巨核细胞、中性粒细胞、嗜碱性粒细胞和成红细胞的分化，而来自MKL的原始细胞显示出向未成熟巨核细胞、成红细胞和少数嗜碱性粒细胞的有限分化。这些结果与培养研究相一致，显示 TMD 母细胞分化为各种类型的血细胞。