Cytological characteristics of cord blood stem cells

脐带血干细胞的细胞学特征

• 批准号: 11670782
• 负责人: EGUCHI Mitsuoki
• 金额: $ 1.73万
• 依托单位: Dokkyo University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670782/
• 关键词: cord blood; stem cell; electron microscopy; leukocyte; cytokine; T lymphocyte; NK cell; 白血病; 培養; ペルオキシターゼ; 骨髄細胞

To identify the characteristics of cord blood cells, we examined their morphological features by electron microscopy and CXCR3 expression on CD4^+ cells and NK cells.Compared with normal peripheral blood cells, cord blood cells frequently showed immature morphology and a unique ultrastructure, such as nuclear pockets in neutrophilis, several crystaloids in a single eosinophilic granulocytes and deformed nuclei in lymphocytes. In contrast to bone marrow cells, cord blood cells yielded a large number of cells of immature myelo-monocytic lineages in cell culture, and demonstrated weaker peroxidase reaction.The percentage of CXCR3 antigen on cord blood and peripheral blood CD4^+ cells was 2.6% and 30.8%, respectively, demonstrating a significant difference. However, that on cord blood and peripheral blood NL cells was not significantly different suggesting milder acute graft-versus-host disease on cord blood stem cell transplantation.

通过电镜观察和CD4^+细胞及NK细胞表面CXCR3的表达，发现脐血细胞与正常外周血细胞相比，形态不成熟，超微结构独特，如嗜酸性粒细胞内有核囊，单个嗜酸性粒细胞内有多个晶体样细胞，淋巴细胞核变形。与骨髓细胞相比，脐血细胞培养中产生大量未成熟的骨髓单核细胞系细胞，过氧化物酶反应较弱，脐血和外周血CD4^+细胞上CXCR3抗原的百分比分别为2.6%和30.8%，差异有显著性。然而，脐带血和外周血中性粒细胞的差异并不显着，这表明脐带血干细胞移植的急性移植物抗宿主病较轻。

项目成果

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