Ultracytochemical Characteristics of Megakaryocytic Leukemia in Children. - Comparison with Adult Cases -.

儿童巨核细胞白血病的超细胞化学特征。

• 批准号: 01570547
• 负责人: EGUCHI Mitsuoki
• 金额: $ 0.83万
• 依托单位: Dokkyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570547/
• 关键词: Megakaryocytic leukemia; Electron microscopy; Platelet peroxidase; 血小板ペルオキシタ-ゼ; ペルオキシダ-ゼ; 電顕細胞化学; 巨核球

Electron microscopic and ultrastructural cytochemical features were compared on 49 children and adults with acute megakaryoblastic leukemia (AMKL) or chronic myelogenous leukemia (CML) in megakaryoblastic crisis. Blast cells from AMKL children with and without Down's syndrome exhibited immature features lacking typical alpha granules and a demarcation membrane system. However, blast cells from AMKL patients with Down's syndrome had more theta, electron-lucent, and basophil-like granules, suggesting that the blast cells had more potential to differentiate into other cell lines than megakaryocytes. The AMKL blast cells of adult patients showed a higher percentage of platelet peroxidase-positivity than other subgroups, and they occasionally contained typical alpha granules and demarcation membrane systems. This indicated that the blast cells of adults with AMKL were more mature than those of children and CML in megakaryoblastic crisis. By electron microscopic analysis, leukemic megakaryoblasts differed between AMKL children with and without Down's syndrome, adults with AMKL, and CML patients in megakaryoblastic crisis.

对49例儿童和成人急性巨核细胞白血病（AMKL）和慢性粒细胞白血病（CML）巨核细胞危象的电镜和超微结构细胞化学特征进行了比较。患有和不患有唐氏综合征的AMKL儿童的母细胞表现出缺乏典型α颗粒和分界膜系统的不成熟特征。然而，患有唐氏综合症的AMKL患者的母细胞具有更多的θ颗粒、电子透明颗粒和嗜碱性粒细胞样颗粒，这表明母细胞比巨核细胞更有潜力分化为其他细胞系。成人患者的AMKL母细胞中血小板过氧化物酶阳性率高于其他亚组，偶尔含有典型的α颗粒和分界膜系统。提示成人AMKL的原始细胞比儿童和CML的巨核细胞危象更为成熟。通过电子显微镜分析，白血病巨核母细胞在伴有和不伴有唐氏综合征的AMKL儿童、AMKL成人和巨核母细胞危象的CML患者之间存在差异。

项目成果

Mitsuoki Eguchi, et al: "Ultrastructural and ultracytochemical differences between trnsient myeloproliferative disorder and megakaryoblastic leukaemia in Down's snyolrome." Brit.J.Haematol.73. 315-322 (1989)

Mitsuoki Eguchi 等人：“唐氏综合症中短暂性骨髓增殖性疾病和巨核细胞白血病之间的超微结构和超细胞化学差异。”

稲葉 俊哉,他: "Ph1 染色体陽性bcr構成陽性の巨核芽球性白血病へ移行した小児骨髄異形成症候群(RAEBT)の1例" 臨床血液. 31. 994-998 (1990)

Toshiya Inaba 等人：“一例儿童骨髓增生异常综合征 (RAEBT) 进展为 Ph1 染色体阳性 BCR 阳性巨核细胞白血病”《临床血液》31. 994-998 (1990)。

Kurosaki et al.: "A case of megakaryoblastic leukemia after recovering from transient abnormal myelopoiesis with mosaic Down's syndrome." Japan J. Pediat. Hematol. 4. 114-119 (1990)

Kurosaki 等人：“一例从伴有马赛克唐氏综合症的短暂性骨髓生成异常恢复后的巨核细胞白血病病例。”

T. Inaba et al.: "Myelodysplastic syndrome in a child which developed into megakaryocytic leukemia with late appearing Phi 1 chromosome and rearrangement of breakpoint cluster region." Japan J. Clin. Hematol. 31. 994-998 (1990)

T. Inaba 等人：“一名儿童的骨髓增生异常综合征，发展为巨核细胞白血病，具有较晚出现的 Phi 1 染色体和断点簇区域的重排。”

江口 光興,他: "白血病の正体を電子顕微鏡で探る" 薬局. 41. 481-487 (1990)

Mitsuoki Eguchi 等人：“使用电子显微镜探索白血病的真实本质”，Pharmacy 41. 481-487 (1990)。