Study on process of rabbit arthritis by immunization of E.coli 0:14 strain

研究了大肠杆菌0:14免疫关节炎家兔的病理变化。经短期（4个月）免疫后，兔膝滑膜上皮细胞增生（32例中有20例，62.5%），下层水肿改变（32例中有16例，50.0%），滑膜浅层纤维蛋白沉积（32例中有19例，59.4%）。长期（8 - 10个月）免疫后，这些发现，增生（36例中29例，80.6%），水肿改变（36例中26例，75.0%）和纤维蛋白沉积（36例中22例，61%）变得更加显著。在此期间，36个膝关节中有10个膝关节出现了瘤样组织（27.8%），36个膝关节中有8个膝关节出现了浅层软骨变性（22.2%）。36例患者中有22例（61.1%）长期免疫后出现淋巴细胞浸润，显著高于32例患者中10例（31.3%）。免疫兔血清中类风湿因子样物质（RFLS）在初始治疗3周后出现。初始治疗15周后，22只兔中有20只（90.9%）RFLS呈阳性。关于RFLS的时间或初始出现有两种模式。免疫8周前出现RFLS组滑膜淋巴细胞浸润的发生率明显高于免疫8周后RFLS阳性组。这些发现表明，家兔的关节炎状况与类风湿关节炎非常相似。该动物模型有助于了解该病的发病机制。

Pathological changes of arthritic rabbits immunized with E.coli 0:14 have been studied. After short term (4 months) immunization, hyperplasia of synovial lining cells (20 knees out of 32, 62.5%), edematous change of the sublining layer(16 knees out of 32, 50.0%) and fibrinoid deposition in the superficial layer of the synovium (19 knees out of 32, 59.4%) observed in the synovium of the rabbits knees. After long term (8 - 10 months) immunization, these findings, hyperplasia (29 knees out of 36, 80.6%), edematous change (26 knees out of 36, 75.0%), and fibrinoid deposition (22 knees out of 36, 61 1%) became more remarkable. In this period, pannus like tissue (10 knees out of 36: 27.8%) and degeneration of superficial layer of cartilage (8 knees out of 36, 22.2%) were also found. In these synovium, lymphocyte infiltration following long term immunization was noted in 22 knees out of 36(61.1%) which was significantly higher (P<0.05) than in 10 knees out of 32(31.3%) following short term immunization. In the serum of the immunized rabbits, rheumatoid factor-like substance (RFLS) appeared 3 weeks after the initial treatment. RFLS was positive in 20 rabbits out of 22 (90.9%) at 15 weeks after the initial treatment. There were two patterns concerning the time or the initial appearance of RFLS. Lymphocyte infiltration in the synovium occurred significantly more frequently in the group in which RFLS appeared before 8 weeks of immunization than in that with RFLS positive after 8 weeks. These findings suggested that arthritic conditions of the rabbits were quite similar to those of rheumatoid arthritis. This animal model is useful in understanding the pathogenesis of this disease.