Development of the enzyme-linked immunosorbent assay kit for sperm-immobilizing antibodies.

开发精子固定化抗体酶联免疫吸附测定试剂盒。

• 批准号: 61570793
• 负责人: SAJI Fumitaka
• 金额: $ 1.47万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570793/
• 关键词: human sperm antigen; sperm cell membrane glycoprotein; sperm immobilization test; 不妊

We have identified a unique sperm cell membrane antigen with a molecular size of 15,000 daltons by using clinically defined serum samples from infertile women with sperm immobilizing antibodies. The stability examination of the antigen against acid, alkali and various proteolytic enzymes indicated that the antigen epitope could be a peptide portion of the glycoprotein. The antisera against this molecule could inhibit sperm motility as well as sperm-egg interaction. In order to develop a rapid reliable assay system for antisperm antibodies, a commercially available enzyme-linked immunosorbent assay (ELISA) kit for antisperm antibodies was compared with standard sperm immobilization test. However, these methods detect a different, though often overlapping spectrum of antibody activity.

我们已经确定了一个独特的精子细胞膜抗原的分子大小为15,000道尔顿，通过使用临床定义的血清样本与精子固定抗体的不孕妇女。抗原对酸、碱和各种蛋白水解酶的稳定性检测表明，抗原表位可能是糖蛋白的肽部分。抗该分子的抗血清能抑制精子运动和精卵相互作用。为了建立一种快速、可靠的抗精子抗体检测系统，将市售抗精子抗体酶联免疫吸附试验（ELISA）试剂盒与标准精子制动试验进行了比较。然而，这些方法检测不同的，尽管经常重叠的抗体活性谱。

项目成果

Fumitaka Saji: Am.J.Rep.Immuool.Microbiol.17. 128-133 (1988)

Fumitaka Saji：Am.J.Rep.Immuool.Microbiol.17。

Fumitaka Saji: "Clinical Evaluation of the ELISA kit for antisperm antibodies." Fertility and Sterility. 50. 644-647 (1987)

Fumitaka Saji：“抗精子抗体 ELISA 试剂盒的临床评估。”

Fumitaka Saji: "Identification of a human sperm antigen to sperm immobilizing antibodies." Acta.Obstet.Gynec.Jpn.39. 1655-1657 (1986)

Fumitaka Saji：“鉴定人类精子抗原与精子固定抗体。”

大橋一友: 日本産科婦人科学会雑誌. 40. (1988)

Kazutomo Ohashi：日本妇产科学会杂志 40。（1988 年）

Fumitaka Saji: Acta.Obstet.Gynec.Jpn.38. 1137-1138 (1986)

Fumitaka Saji：Acta.Obstet.Gynec.Jpn.38。