Development of the enzyme-linked immunosorbent assay kit for sperm-immobilizing antibodies.

我们已经确定了一个独特的精子细胞膜抗原的分子大小为15000道尔顿通过临床确定的血清样本不育妇女的精子固定抗体。抗原对酸、碱和各种蛋白水解酶的稳定性检测表明抗原表位可能是糖蛋白的肽部分。针对该分子的抗血清可以抑制精子运动以及精子与卵子的相互作用。为了开发一种快速可靠的抗精子抗体检测系统，将市售的抗精子抗体酶联免疫吸附试验（ELISA）试剂盒与标准精子固定化试验进行了比较。然而，这些方法检测不同的，虽然经常重叠的抗体活性谱。

We have identified a unique sperm cell membrane antigen with a molecular size of 15,000 daltons by using clinically defined serum samples from infertile women with sperm immobilizing antibodies. The stability examination of the antigen against acid, alkali and various proteolytic enzymes indicated that the antigen epitope could be a peptide portion of the glycoprotein. The antisera against this molecule could inhibit sperm motility as well as sperm-egg interaction. In order to develop a rapid reliable assay system for antisperm antibodies, a commercially available enzyme-linked immunosorbent assay (ELISA) kit for antisperm antibodies was compared with standard sperm immobilization test. However, these methods detect a different, though often overlapping spectrum of antibody activity.