器官培養ならびに移植実験による歯胚の分化と石灰化に関する研究

通过器官培养和移植实验研究牙胚的分化和矿化

• 批准号: 62570812
• 负责人: 名和 橙黄雄
• 金额: $ 0.96万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570812/
• 关键词: 歯胚培養; 歯胚発生; 歯胚の分化; 歯胚の石灰化

1.歯胚の移植実験による結果:14日マウス胎仔の臼歯歯胚を成体マウス脾臓内に移植し, 移植1週間後にコルヒチン0.025mg, 0.05mgを静注した. 12〜24時間後に固定を行い, 電顕的に象牙芽細胞について観察を行った. その結果, 注射12時間後にゴジル領域に著しい変化がみられ, 縞構造を有する線維状物を含有する多数のゴルジ空胞が出現してきた. この縞構造の幅はコラーゲンのバンドの縞と同じ長さであった. このような空胞の出現はコラヒチンの濃度, 処理時間とともに増加の傾向がみられた. コルヒチンは細胞の微細管の形成を障害し, その結果, 細胞分泌の阻害, 食作用の阻害が知られている. これらの結果と本実験の結果を併せて考察してみると, 象牙芽細胞のゴルジ空胞に集積したコラーゲン細線維は象牙芽細胞の貪食作用の結果であるとする説を否定し, コラーゲンの分泌抑制によって生じた可能性を強く支持するものである.2.歯胚の器官培養による結果:17日マウス胎仔の臼歯歯胚を摘出し, 1.25-dihydroxyvitamin VD_3(ビタミンD3)0.1, 1.0, 10mg/mlを含有する培養液で器官培養を行った. 培養2〜4日後に固定して, 内エナメル上皮と歯乳頭細胞の分裂細胞数を計測した. その結果, 培養2日目ではいずれの実験群にも有意差はみられなかったが, 4日目になるとビタミンD_3実験群における内エナメル上皮の分裂細胞は他の実験群に比較して多く, 有意差がみられた. 歯胚に及ぼすビタミンD3の影響については未だ不明の点が多いが, 本研究の結果はビタミンD3が歯胚の発生過程に関与している可能性を示唆するものである.3.骨改造(石灰化)に関する結果:顎骨を固定してleucine aminopepep-tidaseを用いて酵素抗体法を行うと, 破骨細胞の基質とミトコンドリアの特異的反応が観察された.

1. Results: after 14 days of embryo transfer, the adult embryos were implanted into the spleen. After 1 week of transplantation, 0.025mg was detected and 0.05mg was injected intravenously. The train is fixed after 12: 24, and the ivory germ cell of the computer is responsible for monitoring the operation. According to the results of the test, after 12 hours of injection, there was a significant change in the field of treatment, and the product was found to contain a large number of empty cells. In the same way, we made the same picture as we did. There is a change in the temperature of the air cell, and the response rate is higher than that in the normal time. The microtubules of the microtubules form a barrier, the results show that the cellular secretion is blocked, and the food is blocked by food. The results of this experiment showed that the ivory germ cells were divided into two groups: the ivory germ cell, the ivory bud cell, the The results of organ culture were as follows: on the 17th, the embryo was extracted, 1.25-dihydroxyvitamin VD_3 (embryo D3) 0.1,1.0, and 10mg/ml contained tissue culture solution. After 2-4 days of culture, the cells were fixed, the epithelium was fixed, the milk was divided, and the number of cells was calculated. The results showed that on the 2nd day, there were significant differences between the two groups, and on the 4th, the number of epithelial cells in the group was higher than that in the group D _ 3. The results of this study show that the possibility of pregnancy and pregnancy in D3 embryos is not known. 3. The results of bone remodeling (calcinization) test results: the leucine aminopepep-tidase of bone fixation was tested by enzyme antibody method, and the anti-tumor effect of osteoclast was observed.

项目成果

T. Tachibana;N. Fijiwara and T. Nawa: Calcified Tissue International.

T.立花；N.

Y. Sakakura;N. Fujiwara;K. Ishizeki and T. Nawa: Calcified Tissue International.

Y.坂仓；N.

K. Ishizeki;Y. Sakakura;T. Nawa and Y. Harada: Cell and Tissue Research.

K.石关；Y.