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EFFECT OF MECHANICAL STRESS ON PROTEIN SYNTHESIS IN HUMAN PERIODONTAL LIGAMENT CELLS.

机械应力对人牙周韧带细胞蛋白质合成的影响。

基本信息

批准号：
62570847
负责人：
NISHIYAMA Katsuhiro
金额：
$ 1.47万
依托单位：
KANAGAWA DENTAL COLLEGE.
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

The purpose of this study was to characterize the ability of cultured human periodontal ligamentfibroblast(PHLF) in relation to protein and collagen synthesis when mechanical stretching and Epidermal Growth Factor(EGF) were applied. HPLP were plated on 60 mm tissue culture dishes at a density of 1.5 x 10^5 cells/5 ml medium, and cultured for 5 days until confluent state. The cells were then transferred to a medium supplemented with 5 % fetal calf serum containing varying concentrations of EGF and were further cultured for 1 days. During each experimental period culturemedia was changed 2 days and DNA, protein, and cell number were determined. Collagen synthesis was assays ed at 37゜C by the methoddescribed Hata etal. (Hata, R, Biochemi. 19: 169-178,1980). DNA synthesis of HPLF was increased 1.3 fold by stretched and 1.6 fold by stretched (+EGF) as compared to control cells. The p rotein content of cell layers of HPL cells was more significantly increased (P<0.05) by non-stretched than by stretched(+EGF). Non-stretched HPLF indicate that EGF at concentrations of 10-50 ng/ml significantly decreased collagen synthesis in the cells. Collagen synthesisof stretched HPLF increased 5 % by control cells. These results suggestion that the action of EGF and the enhancement of collagen stimulation are increased by mechanical stretching.

本研究的目的是探讨体外培养的人牙周膜成纤维细胞（PHLF）在机械拉伸和表皮生长因子（EGF）作用下蛋白质和胶原合成的能力。将HPLP以1.5 x 10^5个细胞/5 ml培养基的密度接种在60 mm组织培养皿上，培养5天直至融合状态。然后将细胞转移到补充有含有不同浓度EGF的5%胎牛血清的培养基中，并进一步培养1天。在每个实验期间，更换培养基2天，并测定DNA、蛋白质和细胞数。用Hata埃塔尔描述的方法在37 ℃下测定胶原合成艾德。(Hata，R，Biochemi. 19：169- 178，1980）。与对照细胞相比，拉伸的HPLF的DNA合成增加1.3倍，拉伸（+EGF）的HPLF的DNA合成增加1.6倍。未牵张组HPL细胞层蛋白质含量显著高于牵张组（P<0.05）。未拉伸的HPLF表明EGF在10-50 ng/ml的浓度下显著降低细胞中的胶原蛋白合成。拉伸后的HPLF胶原合成比对照组增加5%。这些结果表明，机械拉伸增加了EGF的作用和胶原刺激的增强。