Effect of mastification of derived brain factor

咀嚼对脑内基因表达的影响，咀嚼是指食物粉碎和下颌运动，对脑中枢的机制维持有什么样的影响。在海马区参与回忆学习。用RT-PCR方法检测海马总RNA 1 μ g在各RNA组分中BDNF-mRNA的含量，结果表明，海马总RNA 1 μ g循环数在20个左右，相对稳定。由于EtBr检测的敏感性提高，我能够减少PCR的循环次数。一个饮食组大鼠的BDNF/mRNA水平增加，在大鼠海马中BDNF的mRNA水平随着动量的增加而增加。至于给予daiet的大鼠脑中的基因表达，在海马区和小脑中差异显著，齿状回无法识别下丘脑、饱腹中枢有小的表现。海马区BDNF mRNA表达量的测定与生物图像分析仪20次测定的误差在30%左右。在该测定系统中，在30%左右的再现性中，部分之间的误差变得明显，并且最大紧急。对咀嚼反应的脑区基因表达的定量分析。

Effect of mastification of gene expression in brain.The mastification defined it as diet comminution and jaw movement.I referred to what kind of influence mastification had in mechanism maintenance of brain center. I analyzed in the hippocampus area which participated in anamnesis learning. Total RNA1ug from hippocampus did determination of the quantity of BDNF-mRNA in all RNA fraction by RT-PCR.It was kept by all RNA1ug cycle number around 20got from hippocampus smartly comparatively. It was kept by cycle number around 20 smartly.Because sensibility imprved as for detection by EtBr, I was able to reduce cycle number of PCR.A one of rat of diet increased a level of BDNF/mRNA.m RNA level of BDNF rose with gain of momentum in rat hippocampus.As for gene expression in brain of the rat which daiet was given to, the difference which was significance during hippocampus area and cerebellum, dentate gyrus wasn't recognized. Small manifestation was found in hypothalamus, satiety center. The quantity of mRNA manifestation of BDNF in hippocampus area came with determination with error of around 30% from 20 by using Bio Imaging Analyzer. In this determination system, error between section became clear in reproducibility in around 30% and an emergency by a maximum. Quantitative analysis of gene expression in area in the brain which replied to mastification response.