Investigate a mystification function and life science of a person of advanced age aiming at the health science

以健康科学为目标，研究高龄者的神秘功能和生命科学

• 批准号: 11671960
• 负责人: NISHIYAMA Katsuhiro
• 金额: $ 1.66万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671960/
• 关键词: human; hippocampal; SAMP8 mice; Water mazes; aging; brain; molars; C-Fos; (3)脳; (4)海馬; (5)細胞; (6)神経; (7)痴呆; (8)記憶

The involvement of dysfunctional teeth in senile hippocampal activity was evaluated by examining, in aged SAMP8 mice, the effect of cutting off the upper molars (molarless condition) on hippocampal induction of the protein product, Fos, of the immediate early gene, c-fos, and on spatial performance in a water maze. The molarless condition caused a reduction in the number of Fos-positive cells in the hippocampal CA1 region, in which Fos immunoreactivity was localized in the cell nuclei. This effect was more pronounced the longer the molarless condition persisted. The suppression of both learning ability and Fos induction in the CA1 induced by the molarless condition was considerably reduced by restoring the lost molars with artificial crowns. Taken together with the plethora of research showing a relationship between stress, aging and hippocampal function and our past findings [Brain Res. 1999 ; 826 : 148-53 ; Behave. Brain Res. 2000 ; 108 : 145-55 ; Exp. Gerontology. 2001 ; 36 : 283-95], the present results suggest the detrimental effects of a reduction in chewing on hippocampal processing in aged SAMP8 mice that would be linked with stress induced by the molarless condition.

通过检查老年SAMP 8小鼠切断上磨牙（无磨牙条件）对海马诱导即刻早期基因（c-fos）的蛋白产物Fos和水迷宫中空间表现的影响，评估了老年海马活动中功能障碍牙齿的参与。无磨牙条件下引起的Fos阳性细胞在海马CA 1区，其中Fos免疫反应位于细胞核中的数量减少。无磨牙状态持续的时间越长，这种影响就越明显。用人工冠修复丢失的磨牙，可以大大减少无磨牙条件下对CA 1区学习能力和Fos诱导的抑制。结合大量显示压力、衰老和海马功能之间关系的研究以及我们过去的发现[Brain Res. 1999 ; 826：148-53 ; Behave. Brain Res. 2000 ; 108：145-55 ; Exp.老年学二○ ○一年;第三十六章：283-95]，本结果表明咀嚼减少对老年SAMP 8小鼠海马加工的有害影响，这与无磨牙条件诱导的应激有关。

项目成果

Watanabe K, Nishiyama K: "Inpairement of spatial memory and changed in astroglial responses following loss of molar teeth in aged SAMP8 mice"Behav Brain Res. 108. 145-155 (2000)

Watanabe K、Nishiyama K：“老年 SAMP8 小鼠臼齿缺失后空间记忆受损和星形胶质细胞反应发生变化”Behav Brain Res。

Karasawa K, Nishiyama K: "Age-associated change in the dopamine systhesis as dertmined by GTP ciclohydrolase I inhibitor in the brain of senescnce-accelerated mouse-prone inbred strains(SAMP8)"Neuroscience Research. 35. 31-36 (1999)

Karasawa K、Nishiyama K：“衰老加速小鼠近交系 (SAMP8) 大脑中 GTP 环水解酶 I 抑制剂确定的多巴胺合成与年龄相关的变化”神经科学研究。

Onozuka M, Nishiyama K: "Reduced mastication stimulates impairment of spairal memory and degeneration of hipocampal neuroms in aged SAMP8 mice"Brain Res. 826. 148-153 (1999)

Onozuka M、Nishiyama K：“咀嚼减少会刺激老年 SAMP8 小鼠的配对记忆受损和海马神经元退化”Brain Res。