In vitro evolution of more thermostable dextranases for the Australian sugar industry

澳大利亚制糖业更耐热的葡聚糖酶的体外进化

• 批准号: LP0453776
• 负责人: Em/Prof Peter Bergquist
• 金额: $ 16.71万
• 依托单位: Macquarie University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2004
• 资助国家: 澳大利亚
• 起止时间: 2004-01-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP0453776
• 关键词: vitro; evolution; more; thermostable; dextranases

Dextrans are polysaccharides that adversely affect the productivity of sugarcane mills. The Australian sugar industry currently imports dextranases to deal with this problem but they are not heat stable so the mills have run at lower temperatures than optimal. We have isolated dextranases from thermophilic microorganisms and aim to improve their performance by generating superior heat-stable dextranases using in vitro evolution, creating new activity levels by random mutation and recombination. The research will be significant in providing novel enzymes for domestic use, allowing import substitution and an outcome will be the development of a product with export potential.

葡聚糖是对甘蔗米尔斯的生产率有不利影响的多糖。 澳大利亚制糖业目前进口葡聚糖酶来解决这个问题，但它们不是热稳定的，所以米尔斯在低于最佳温度的温度下运行。我们已经从嗜热微生物中分离出葡聚糖酶，旨在通过体外进化产生上级热稳定葡聚糖酶，通过随机突变和重组创造新的活性水平，从而提高其性能。该研究将在提供国内使用的新型酶方面具有重要意义，允许进口替代，其结果将是开发具有出口潜力的产品。