Genetic modification and lyophilisation of microorganisms for the generation of bacteriological internal quality controls.

微生物的基因改造和冻干，用于细菌学内部质量控制。

• 批准号: LP0347861
• 负责人: Em/Prof Peter Bergquist
• 金额: $ 12.57万
• 依托单位: Macquarie University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-08-19 至 2006-08-16
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP0347861
• 关键词: Genetic; modification; lyophilisation; microorganisms; generation

The development of internal quality control micro-organisms in precise numbers is necessary for the evolution of standard methodology in microbiology, which until now, remains obsolete, because it relies on inaccurate methods to produce quantitative and qualitative results. The research described here is largely based on molecular techniques to genetically tag micro-organisms with fluorescent proteins and pigment producing enzymes, and on the manipulation of growth and storage conditions to maximize the survival of micro-organisms during lyophilisation. Successful completion and application of the proposed project through existing patents owned by BTF, will revolutionise the way microbiological tests are performed worldwide.

精确数量的内部质量控制微生物的发展对于微生物学标准方法的发展是必要的，到目前为止，微生物学标准方法仍然过时，因为它依赖于不准确的方法来产生定量和定性结果。这里描述的研究主要是基于分子技术，用荧光蛋白和色素产生酶对微生物进行遗传标记，以及操纵生长和储存条件，以最大限度地提高微生物在冻干过程中的存活率。通过BTF拥有的现有专利，该项目的成功完成和应用将彻底改变全球微生物检测的方式。