International Joint Research on Pineal Photoereception

松果体感光国际联合研究

• 批准号: 63044059
• 负责人: MORITA Yukitomo
• 金额: $ 7.68万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63044059/
• 关键词: Pineal Organ; Antarctic Fish; UV-receptor; Deep-brain Photoreceptor; Electrophysiology; Immunocytochemistry; Lamprey; Electric Fish

Environmental photic information regulates the function of pineal organs. In order to elucidate basic mechanisms of pineal functions, the international and interdisciplinary cooperative research was undertaken between our laboratory and the scientists from Germany, U.S.A., New Zealand and India. Main findings are as follows.1. Electrophysiological and immunocytochemical study of pineal organs and retina of the Antarctic fishes, Pagothenia and Trematomus, Showed that they are well-developed photosensitive organs.2. UV-receptor was found electrophysiologically in the lamprey's pineal, showing the peak sensitivity at 380 nm.3. Application of NADPH-diaphorase staining demonstrated 4 types of pineal neurons, which could be subdivided into 8 groups. Double immunostaining with antisera against seretonin and rod-opsin was successful. A new cell-type with rob-opsin positivity without processes was found. Cone-opsin immunoreactivity suggested the presence of deep-brain photoreceptors in the paraventricular organ of lamprey.4. Amazonian electric fish had pineal organs. In the retina of electric eel, rod-opsin immunoreactivity was shown in outer segments of visual cells, but no serotonin-positive amacrine cells were demonstrated.5. Pineal photoreceptors of the lamprey were studied electrophysiologically whether they are sensitive to magnetic field.Intracellular recording revealed no change in the resting membrane potential and in photic responses.

环境光信息调节松果体器官的功能。为了阐明松果体功能的基本机制，本实验室与来自德国、美国、新西兰和印度。主要研究结果如下：1.对南极鱼类（Pagothenia和Trematomus）的松果体和视网膜进行了电生理和免疫细胞化学研究，结果表明：它们是发达的感光器官.在七鳃鳗的松果体中发现了紫外线受体，在380 nm处显示出最高的敏感性。NADPH黄递酶染色显示松果体神经元有4种类型，可分为8组。用抗seretonin和视杆细胞视蛋白的抗血清进行双重免疫染色是成功的。发现了一种新的视锥蛋白阳性细胞类型，无突起，视锥蛋白免疫反应表明七鳃鳗室旁器中存在脑深部光感受器.亚马逊电鱼有松果体器官。在电鳗视网膜中，视杆细胞外节有视蛋白免疫反应阳性细胞，但无长突细胞呈阳性.研究了七鳃鳗松果体光感受器对磁场是否敏感的电生理学特征，细胞内记录结果表明，松果体光感受器的静息膜电位和光反应没有变化。

项目成果

Uchida,K.: "Intracellular responses from UVーsensitive cells in the photosensory pineal organ." Brain Research. 534. 237-242 (1990)

Uchida, K.：“光感觉松果体器官中紫外线敏感细胞的细胞内反应。”534. 237-242 (1990)。

Samejima,M.: "Photoreceptor cells and neural elements with long axonal processes in the pineal organ of the lamprey,Lampetra japonica,identified by use of the horseradish peroxidase method." Cell and Tissue Research. 258. 219-224 (1989)

Samejima，M.：“七鳃鳗松果体中具有长轴突的感光细胞和神经元，Lampetra japonica，通过使用辣根过氧化物酶方法进行鉴定。”

Samejima, M.: "Photoreceptor cells and neural elements with long axonal processes in the pineal organ of the lamprey, Lampetra japonica, identified by use of the horseradish peroxidase method." Cell and Tissue Research. 258. 219-224 (1989)

Samejima, M.：“七鳃鳗松果体中具有长轴突的感光细胞和神经元件，Lampetra japonica，通过使用辣根过氧化物酶方法进行鉴定。”

Morita,Y.: "Multiplicity of electrophysiological and immunocytochemical properties in the pineal photosensory system." Advances in Pineal Research. 3. 43-48 (1989)

Morita，Y.：“松果体光感觉系统中电生理学和免疫细胞化学特性的多样性。”

Tamotsu, S.: "Blue sensitive visual pigment and photoregeneration in pineal photoreceptors measured by high performance liquid chromatography." Comparative Biochemistry and Physiology. 96B. 487-490 (1990)

Tamotsu, S.：“通过高效液相色谱法测量松果体感光器中的蓝色敏感视觉色素和光再生。”