Photopigments in photosensory pineal organ
松果体感光器官中的感光色素
基本信息
- 批准号:61570040
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1986
- 资助国家:日本
- 起止时间:1986 至 1988
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Photopigments of the direct light-sensitive pineal organ of lamprey, Lampetra japonica, were analyzed by high performance liquid chromatography (HPLC) and by immunohistochemistry.(1) Retinal and 3-dehydro-retinal were identified by HPLC, that means, the presence of the mixture of vitamin-A1-based pigment and vitamin-A2-based pigment was confirmed.(2) The ratio of all-trans isomer to 11-cis isomer was higher than that of retina.(3) Photopigment sensitive to short wavelength light was confirmed by the finding that the ratio of all-trans isomer to 11-cis isomer was reversed when the sample was irradiated by the short wavelength light after the orange light irradiation. The finding coincides the high sensitivity of pineal orgen to short wavelength light demonstrated electrophysiologically.(4) Spectral sensitivity was measured intracellularly in the pineal photoreceptor cells of adult and larval lamprey. The spectral sensitivity curve of larval pineal has a peak at 505 nm and that of adult was 20 nm shifted toward longer wavelength. This suggests the conversion of the visual pigment during the metamorphosis.(5) Opsin-positive photoreceptor cells were observed immunohistochemically. The reactivity was restricted in the outer segment of the photoreceptor.(6) Visinin-positive photoreceptor cells were observed in the periphery of the pineal. Gradation of visinin reactivity suggests the different types of photoreceptors in the pineal.(4) photoreceptor cells were also confirmed inM parapineal organ immunohistochemically.
采用高效液相色谱法和免疫组织化学法对七鳃鳗直接感光松果体的光色素进行了分析。(1)通过HPLC鉴定了视黄醇和3-脱氢-视黄醇,这意味着确认了基于维生素A1的色素和基于维生素A2的色素的混合物的存在。(2)全反式异构体与11-顺式异构体的比例高于视网膜。(3)通过在橙子光照射后用短波长光照射样品时,全反式异构体与11-顺式异构体的比率反转的发现,证实了对短波长光敏感的光颜料。这一发现与松果体对短波长光的电生理学高敏感性相一致。(4)光谱敏感性测定细胞内松果体感光细胞的成年和幼虫七鳃鳗。幼虫松果体的光谱敏感曲线在505 nm处有一个峰值,成虫松果体的光谱敏感曲线向长波方向移动了20 nm。这表明在变态过程中视觉色素的转换。(5)光化学观察到视蛋白阳性的感光细胞。反应性仅限于光感受器的外节。(6)在松果体周围可见视见素阳性的感光细胞。视蛋白反应性的分级表明松果体中存在不同类型的光感受器。(4)光化学方法也证实了M松果体旁器中存在感光细胞。
项目成果
期刊论文数量(37)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Samejima, M.; Morita, Y.: "External sodium ions are required for the light response in pineal photoreceptors." Vision Research. 28. 251-258 (1988)
鲛岛,M.;
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Morita, Y.; Samejima, M.; Tamotsu, S.: "Response pattern and neuronal network of photosensory pineal organs." Archives of Histology and Cytology. 52. (1989)
森田,Y.;
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Tamotsu,S.et al.: J.Physiol.Soc.Jpn.(abs.). 84. 297 (1986)
Tamotsu,S.et al.:J.Physiol.Soc.Jpn.(abs.)。
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Morita, Y.; Uchida, K.: "Photosensory mechanisms of pineal organ. (in Japanese)" Seitaino Kagaku. 38. 316-321 (1987)
森田,Y.;
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MORITA Yukitomo其他文献
MORITA Yukitomo的其他文献
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{{ truncateString('MORITA Yukitomo', 18)}}的其他基金
Serum melatonin rhythm in demented patients
痴呆患者血清褪黑素节律
- 批准号:
07670081 - 财政年份:1995
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
International Joint Research on Pineal Photoereception
松果体感光国际联合研究
- 批准号:
63044059 - 财政年份:1988
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for international Scientific Research
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