ION AND ELECTRON TRANSPORT ACROSS ENZYME IMMOBILIZED BIPOLAR MEMBRANE

跨酶固定双极膜的离子和电子传输

• 批准号: 63550660
• 负责人: TANIOKA Akihiko
• 金额: $ 1.15万
• 依托单位: TOKYO INSTITUTE OF TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63550660/
• 关键词: Membrane; Enzyme; Bipolar; Urea; Urease; Coupling; Ion; Weak Electrolyte; 酵素固定化; バイポーラー膜; カップリング; 荷電膜; アニオン交換膜; カチオン交換膜; ウレア; アレアーゼ

The substance flow including electron across a membrane is induced by the difference of electrochemical potential, the flow of other substances and the chemical reaction. It is well known that no flow should be coupled with chemical reaction if the system is composed of symmetric structure because chemical reaction is scalar. lt,however,could be coupled if the structure of the system is asymmetric.An enzyme-immobilized bipolar membrane, which has an asymmetric structure consisting of positively and negatively charged layers on each side of the membrane and is containing enzyme between them, was studied. The coupling mechanism of the transport and chemical reaction was discussed using urea-urease system. In course of this analysis, we found that the transport mechanism of weak electrolyte across charged membrane was not systematized yet theoretically and experimentally. To clarify these problems we firstly investigate the permeability coefficients of Na_2SO_3 as a function of concentration of external solution of the membrane. Secondary the transport phenomena of weak electrolyte across charged membrane are investigated theoretically considering the result of Na_2SO_3 permeation, where the dissociation equilibrium constant has to be included in the equation. The theoretical results well explained coupling phenomena of transport and chemical reaction qualitatively.

包括电子在内的物质跨膜流动是由电化学电位差、其他物质的流动和化学反应引起的。由于化学反应是标量的，所以当系统是对称结构时，流动不应与化学反应耦合。本文研究了一种固定化酶的双极膜，它具有不对称的结构，在膜的两侧各有一层带正电荷和负电荷的层，在它们之间含有酶。利用尿素-尿素酶体系探讨了尿素在土壤中的迁移与化学反应耦合机理。在分析的过程中，我们发现弱电解质在荷电膜中的传输机理在理论和实验上都还没有系统化。为了澄清这些问题，我们首先研究了Na_2SO_3的渗透系数作为膜外溶液浓度的函数。其次，考虑Na_2SO_3渗透的结果，从理论上研究了弱电解质在荷电膜中的迁移现象，其中离解平衡常数必须包含在方程中。理论结果很好地定性解释了输运与化学反应的耦合现象。

项目成果

Y.Yokoyama: "Enzyme Immobilization in an Asymmetric Charged Membrane" J.Membrane Sci.38. 223-236 (1988)

Y.Yokoyama：“不对称带电膜中的酶固定化”J.Membrane Sci.38。

Y.Nakagawa: "A Study on Coupling of Transport and Chemical Reaction across the Charged Membrane" Sen-i Gakkaishi. (1990)

Y.Nakakawa：“带电膜上传输和化学反应耦合的研究”Sen-i Gakkaishi。

Y. Yokoyama, A. Tanioka and K. Miyasaka: "Preparation of a Single Bipolar Membrane by Plasma-Induced Graft Polymerization" J. Membrane Sci., 43 165-175 (1989).

Y. Yokoyama、A. Tanioka 和 K. Miyasaka：“通过等离子体诱导接枝聚合制备单双极膜”J. Membrane Sci.，43 165-175 (1989)。

Y. Nakagawa, A. Tanioka and K. Miyasaka: "A Study on Coupling of Transport and Chemical Reaction across the Charged Membrane" Sen-i Gakkaishi (1990).

Y. Nakakawa、A. Tanioka 和 K. Miyasaka：“带电膜传输和化学反应耦合的研究”Sen-i Gakkaishi (1990)。

Mitsuru Higa: "An Experimental Study of Ion Permeation Across a Charged Membrane in Multi component Ion Systems as a Function of Membrane Charge Density" J.Membrane Science.

Mitsuru Higa：“作为膜电荷密度函数的多组分离子系统中带电膜离子渗透的实验研究”J.Membrane Science。