Chromatographic Separation of Proteins Using chitosan Ion Exchangers

利用壳聚糖离子交换剂对蛋白质进行大规模色谱分离，在技术上和经济上都是可行的。壳聚糖离子交换剂是由节肢动物壳制成的，成本很低。具体结论如下：1。牛血清白蛋白（BSA）在交联壳聚糖上的吸附在含盐溶液上在技术上是可行的。当无机电解质在溶液中共存时，电解质平衡项受盐浓度和pil的影响。吸附在树脂上的BSA可以用pH=9.2的缓冲液洗脱。强碱交联壳聚糖在除缓冲液外不含其他无机电解质的情况下，对牛血清白蛋白的吸附效果良好。平衡等温线受pH值影响较大。PH=7时，吸附量最大。吸附在树脂上的牛血清白蛋白用NaCl水溶液洗脱。BSA的颗粒内有效扩散率受BSA浓度和ph的影响，当BSA浓度低于1 g/dm^3时，吸收数据与表面和孔隙扩散的平行传输机制一致。当BSA浓度大于1 g/dm^3时，孔隙扩散为速率控制步骤，收缩岩心模型与实验数据吻合较好。测定了突破曲线和洗脱曲线。由于离子交换粒子很硬，所以即使床很长，溶液也能很好地流过床。实验突破曲线与我们提出的解析解吻合较好[Yoshida et al；化学。Eng。科学。[j].农业科学，1999,14（1984）。由突破曲线测定的颗粒内扩散系数与浅层法测定的结果一致。

Large-scale chromatographic separation of proteins using the chitosan ion exchangers, which may be made very cheaply from the shells of arthropods, appears technically and economically feasible. Details of the conclusions are as follows :1. The adsorption of bovin serum albumin (BSA) on the crosslinked chitosan appears technically feasible for salt-containing solutions. When inorganic elecLrolyLes coexisted in the solulioii, Llie equilibrium isol[ierms were liLLIe affected boti by the concentration of the salt and by pil. BSA adsorbed on the resin can be eluted by buffer solution of pH=9.2. The strong base crosslinked chitosan adsorbed BSA well when there were no inorganic electrolytes except for buffer in the solution. The equilibrium isotherms were affected by pH considerably. When PH=7, the amount of adsorption was largest. BSA adsorbed on the resin- was eluted by NaCl aqueous solution.3. The intraparticle effective diffusivity of BSA was affected by the concentration of BSA and pH. When the concentration of BSA is lower than I g/dm^3, the uptake data agreed well with the parallel transport mechanism of surface and pore diffusions. When the concentration of BSA is higher than I g/dm^3, the pore diffusion was the rate controlling step and shrinking core model agreed well with the data.4. Breakthrough curves and elution curves were measured. Since the ion exchange particles are hard, the solution flowed through the bed well even when the bed was long. The experimental breakthrough curves agreed well with the analytic solution presented by us[Yoshida et al ; Chem. Eng. Sci., 39, 1489(1984)]. The intraparticle diffusivities determined from the breakthrough curves agreed with those determined by the shallow bed method.