GENETIC STUDY ON ALLOTETRAPLOIDY GOLDFISH AND CRUSSIAN CARP BY MATING EXPERIMENTS

异源四倍体金鱼和鲫鱼的交配遗传研究

• 批准号: 07660232
• 负责人: KIJIMA Akihiro
• 金额: $ 1.34万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660232/
• 关键词: MATING EXPERIMENT; GENETIC CONTROL; ISOZYMES; GOLDFISH; CRUSSIAN CARP; GENETIC MARKER; キンブナ; 異質4倍体; mtDNA

Since goldfish and crussian carp would have been evolved from allo-tetraploid origin, isozyme patterns appeared with complexities and genetic control of them could not be clarified yet. Then genetic analyzes for inter- and intra-specific relationship has not been carried out deeply, yet. In the present study, convenient usable genetic markers for population study are chosen and the genetic control of chosen markers has been analyzed by mating experiments. As the results, (1) Nuclear DNA and mitochondrial DNA are usable genetic markers for individual and population study, but not convenient compared with isozymes. (2) Clear bands of isozymes in 10 enzymes appeared by starch gel electrophoresis, easily. (3) Genetic control of 21 out of 26 isozymic loci control for 10 enzymes has been demonstrated in gold fish and crussian carp by 3 and 11 combinations of pair maiting experiments, respectively. (4) But further combination of mating are needed for clarify the genetic control of isozymes in Gpi-2 and Ldh-1,2,3,4 loci. (5) Genetic variability is higher in natural population of crussian carp than in artificial strains of goldfish, but not fixed in any strain of goldfish which has been selected strictly. (6) Levels of genetic differentiation are estimated as local race level within strains of goldfish and within population of natural crussian carp, and as subspecies level between goldfish and natural crussian carp.

由于金鱼和鲫鱼可能是从异源四倍体起源进化而来的，因此它们的同工酶谱具有复杂性，其遗传控制尚不清楚。而种间和种内亲缘关系的遗传分析还没有深入开展。本研究选择了便于群体研究的遗传标记，并通过交配试验分析了标记的遗传控制作用。结果表明：（1）核DNA和线粒体DNA是研究个体和群体的有用遗传标记，但与同工酶相比，它们并不方便。(2)淀粉凝胶电泳10种酶的同工酶谱带清晰。(3)通过3个和11个配对试验组合，分别证实了金鱼和鲫鱼10种酶的26个同工酶位点中的21个位点具有遗传控制。(4)但Gpi-2和Ldh-1、2、3、4位点的遗传控制还有待于进一步的交配组合。(5)鲫鱼自然群体的遗传变异性高于金鱼人工品系，但在经过严格选择的金鱼品系中并不固定。(6)遗传分化水平估计为金鱼品系内和天然鲫鱼种群内的地方小种水平，以及金鱼和天然鲫鱼之间的亚种水平。

项目成果

Takase T.: "Genetic polymorphism and isozyme patterns of 6-phosphogluconate dehydrogenase in gold fish and crucian carp" Tohoku J. Agr. Res.47(3,4). (1997)

Takase T.：“金鱼和鲫鱼6-磷酸葡萄糖酸脱氢酶的遗传多态性和同工酶模式”Tohoku J. Agr。

Takase,T.: "Genetic polymorphism and isozyme patterns of 6-phosphogluconate dehydrogenase in goldfish and crussian carp" Tohoku J.Agr.Res.47. 67-75 (1997)

Takase,T.：“金鱼和鲫鱼6-磷酸葡萄糖酸脱氢酶的遗传多态性和同工酶模式”Tohoku J.Agr.Res.47。

Takase, T.et. al.: "Genetic polymorphism and isozyme patterns of 6-phosphogluconate dehydrogenase in goldfish and crussian carp" Tohoku J.Agr. Res.47 (3,4). 67-75 (1997)

高濑，T.et。