Breeding structure in fish population by RFLP analysis of mitochondrial DNA

线粒体DNA的限制性片段长度多态性分析是一种适合于估计养殖群体母系数量的方法，因为它是母性遗传的，在动物中是一个非重组基因组(最常见的是16-18Kbp)，单倍型的数量直接代表母系的数量等。(2)用15种6碱基识别的限制性内切酶对两个养殖群体的5种单倍型进行了检测。(3)用双酶切法绘制单倍型限制性内切酶图谱，确定了鲤鱼的33个限制性内切酶切点。(4)从限制性内切酶检测结果来看，线粒体DNA的变异可能是由于碱基的简单替换所致。根据6个碱基识别的限制性内切酶对马哈鱼养殖群体mtDNA的RFLP分析结果，(5)在4个群体的59个个体中观察到13种mtDNA单倍型。(6)非选择群体的核苷酸多样性在0.00170~0.00253之间，而选择的Amago-Parr群体未观察到变异。(7)结果表明，Amago-Parr群体由一个母系组成，而其他群体由多个母系组成。

The RFLP(restriction fragment length polymorphism)analysis of the mitochondrial DNA(MTDNA)genome is a suitable method for estimating the number of maternal lines in cultured stocks because : maternal inheritance, it is a nonrecombining genome(apploximately 16-18Kbp)in animals, the number of haplotypes represents the number of maternal lines directly and so on.In the present study, I : (l)modification of the method of rapid isolation of mtDNA from carp and masu salmon complex is successfully carried out by alkaline treatment with glassailk. (2)The 5 haplotypes were detected in the two cultured stocks of carp by using 15 kinds of 6-base recognizing restriction endonucleases. (3)Restriction maps of the haplotypes were made up by double-digestion of endonucleases and then the 33 restriction sites were defined in the carp. (4)From the results of restriction naps, the variation of mtDNA is considered to be occurred by the simple substitution of bases. II : Based on the results of RFLP analysis of mtDNA using six 6-base recognized restriction endonuclease in the cultured stocks of masu salmon complex, (5)13 mtDNA haplotypes were observed among 59 individuals of the 4 stocks. (6)The nucleotide diversity within the non-selected stocks ranged from 0.00170 to 0.00253, while no variation was observed in selected amago-parr stock. (7)The results suggest that the amago-parr stock consists of one maternal line while the other stocks consist of several maternal lines.