Studies on the body fluids and tissue isoenzyme diagnosis in domestic animals by using multivariate analysis

1)正常奶牛肝脏中LDH 1 ~ LDH 5的平均值分别为31.7%、24.8%、27.3%、12.8%和3.3%。在肝脏水变性的情况下，与正常奶牛相比，模式显示h型异酶增加。肝内LDH 1降低，LDH 2升高，呈脂肪变性。在充血肝中，LDH 1降低，m型同工酶升高。坏死灶肝脏h型同工酶降低，m型同工酶升高。采用判别分析比较各病变组同工酶带谱。除充血肝与脂肪肝（72%）、充血肝与坏死肝（79%）外，其余病变组的总正确率均在80%以上。提示缺氧引起的肝细胞功能性损伤可能是影响肝脏LDH同工酶谱的重要因素。2)检测ICR小鼠、Wistar大鼠、豚鼠和金仓鼠组织中LDH和CPK同工酶水平。获得的组织有：肝脏、胰腺、胃、小肠、心脏、股肌、子宫、肾皮质、脾脏、淋巴结、大脑、脊髓和红细胞。在小鼠、豚鼠和仓鼠组织中，LDH同工酶呈5条条带。在大鼠体内，LDH呈现4条条带。在实验动物的组织中，CPK同工酶呈现出三个条带。在仓鼠中，MM部分被分成两部分。该技术可用于阐明近交实验动物的个体发育和系统发育分类。3)从实验诱导的肾损伤结果和奶牛的临床病例来看，尿中乳酸脱氢酶同工酶谱与肾皮质和肾髓质的相关性较好。

1) The mean values of LDH 1 through LDH 5 in the livers of normal cows were 31.7%, 24.8%, 27.3% 12.8% and 3.3%, respectively. In the cases with hydropic degeneration of the liver, the patterns revealed an increase of H-type iso- enzymes as compared to the normal cows. The patterns showed a decrease of LDH 1 and an increase of LDH 2 in the liver showing fatty degeneration. In congestic livers, LDH 1 decrease and M-type isoenzymes increased. In the liver showing necrotic foci, H-type isoenzymes decreased and M-type isoenzyme increased. Isoenzyme band patterns for each diseased groups were compared using discriminant analysis. The total correct rate among diseased groups were more than 80%, except between the congestic liver and the fatty liver (72%), and the congestic liver and the necrotic liver (79%). It was suggested that functional hepatocellular damage due to anoxia might be an important factor to affect the liver LDH isoenzyme patterns.2) LDH and CPK isoenzymes in the tissues of the ICR mouse, Wistar rat, guinea pig and golden hamster were examined. The tissues obtained were as follows: liver, pancreas, stomach, small intestine, heart, femoral muscle, uterus, renal cortex, spleen, lymph node, cerebrum, spinal cord and erythrocyte. In the tissues of the mouse, guinea pig and hamster, LDH isoenzymes showed five bands. In the rat, LDH showed four bands. In the tissues of the each laboratory animals, CPK isoenzyme showed three bands. In the hamster, the MM fraction was separated in two fractions. This technique may be applied to elucidate ontogenetic and phylogenetic classification of inbred laboratory animals.3) The adequate correlation of LDH isoenzyme patterns between the urine and the renal cortex and medulla was estimated from the results of the experimental induced renal damage and the clinical cases in the cows.