Studies on the body fluids and tissue isoenzyme diagnosis in domestic animals by using multivariate analysis

1)正常牛肝脏LDH 1 ~ LDH 5的平均值分别为31.7%、24.8%、27.3%、12.8%和3.3%。在肝脏水肿变性的病例中，与正常奶牛相比，模式显示H型同工酶增加。肝脏LDH 1减少，LDH 2增加，表现为脂肪变性。肝组织LDH 1降低，M型同工酶升高。在显示坏死灶的肝脏中，H型同工酶减少，M型同工酶增加。用判别分析比较各组同工酶带型。除肝硬化与脂肪肝、肝硬化与坏死肝的诊断正确率分别为72%、79%外，其他各组的诊断正确率均在80%以上。提示缺氧性肝细胞功能损害可能是影响肝脏LDH同工酶谱的重要因素。2）对ICR小鼠、Wistar大鼠、豚鼠和金黄地鼠的组织LDH和CPK同工酶进行了检测。获得的组织如下：肝脏、胰腺、胃、小肠、心脏、股肌、子宫、肾皮质、脾脏、淋巴结、大脑、脊髓和红细胞。在小鼠、豚鼠和仓鼠的组织中，LDH同工酶均呈五条带。大鼠LDH呈四条带。各实验动物组织中CPK同工酶均呈三条带。在仓鼠中，MM组分分离为两个组分。该技术可用于阐明近交系实验动物的个体发育和系统分类。3）根据实验性肾损伤和临床病例的结果，估计了尿和肾皮质和髓质之间LDH同工酶谱的适当相关性。

1) The mean values of LDH 1 through LDH 5 in the livers of normal cows were 31.7%, 24.8%, 27.3% 12.8% and 3.3%, respectively. In the cases with hydropic degeneration of the liver, the patterns revealed an increase of H-type iso- enzymes as compared to the normal cows. The patterns showed a decrease of LDH 1 and an increase of LDH 2 in the liver showing fatty degeneration. In congestic livers, LDH 1 decrease and M-type isoenzymes increased. In the liver showing necrotic foci, H-type isoenzymes decreased and M-type isoenzyme increased. Isoenzyme band patterns for each diseased groups were compared using discriminant analysis. The total correct rate among diseased groups were more than 80%, except between the congestic liver and the fatty liver (72%), and the congestic liver and the necrotic liver (79%). It was suggested that functional hepatocellular damage due to anoxia might be an important factor to affect the liver LDH isoenzyme patterns.2) LDH and CPK isoenzymes in the tissues of the ICR mouse, Wistar rat, guinea pig and golden hamster were examined. The tissues obtained were as follows: liver, pancreas, stomach, small intestine, heart, femoral muscle, uterus, renal cortex, spleen, lymph node, cerebrum, spinal cord and erythrocyte. In the tissues of the mouse, guinea pig and hamster, LDH isoenzymes showed five bands. In the rat, LDH showed four bands. In the tissues of the each laboratory animals, CPK isoenzyme showed three bands. In the hamster, the MM fraction was separated in two fractions. This technique may be applied to elucidate ontogenetic and phylogenetic classification of inbred laboratory animals.3) The adequate correlation of LDH isoenzyme patterns between the urine and the renal cortex and medulla was estimated from the results of the experimental induced renal damage and the clinical cases in the cows.