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STUDY ON CLINICAL EVALUATION OF SERUM AND URINE ENZYMES IN ORDER TO IMPROVE ACCURACYIN VETERINARYMEDICINE

血清和尿液酶的临床评价研究以提高兽药准确性

基本信息

批准号：
09660335
负责人：
YASUDA Jun
金额：
$ 2.18万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Serum alkaline phosphates (ALP) and adenosine deaminase (ADA) were evaluated in the cases of liver and tumor diseases in cows and dogs by using an auto analyzer. We had established a quantification method for that ALP isoenzymes in the serum combined with white germ lectin precipitation and chemical inhibition of bone ALP (BALP) and liver ALP (LALP) isoenzymes with levamisole to quantify BALP and corticosteroid-induced ALP (CALP), respectively. Because CALP and BALP were known, LALP was calculated from the equation : ALP=BALP+LALP+CALP.We discussed with the relationship between serum ALP isoenzymes and the degree of glucocorticoid induced hepatopathy. The changes in ALP, LALP and CALP were compared to changes in vacuolization of the hepatic parenchyma CALP elevation was found to directly correlate with the severity of vacuolization unlike LALP that increased in all cases treated with glucocorticoids and was non-specific to the degree of vacuolization. Bovine serum and liver tissues which were diagnosed as being from abnormal livers were investigated to determine the relationship between the serum ADA and the degree of mononuclear cell infiltration in the liver. The serum ADA was elevated accompanied by an increase of inflammatory cell infiltration in the liver. Thus, serum ADA analysis of cows might be a predictive index of inflammatory change in the liver. It was suggested that the ADA activity in the canine serum is insatable in the condition of preservation. Serum ADA in cases of canine lymphoma, leukeamia, liver tumor and meningioma increased before chemotherapy. The ADA levels tended to decrease after chemotherapy. Its fluctuation has different clinical significance to that of ALP or lactate dehydrogenase (LDH) in the cases of tumor disorders. We suggest that ADA analysis in dogs may provide one predictive index for tumor behavior.

采用自动分析仪测定奶牛和犬肝脏疾病和肿瘤患者血清碱性磷酸盐（ALP）和腺苷脱氨酶（ADA）。建立了血清中ALP同工酶的定量方法，结合白芽凝集素沉淀和左旋咪唑化学抑制骨ALP （BALP）和肝ALP （LALP）同工酶，分别定量BALP和皮质类固醇诱导的ALP （CALP）。因为CALP和BALP是已知的，所以LALP的计算公式为：ALP=BALP+LALP+CALP。我们讨论了血清ALP同工酶与糖皮质激素所致肝病程度的关系。将ALP、LALP和CALP的变化与肝实质空泡化的变化进行比较，发现CALP升高与空泡化的严重程度直接相关，而LALP在所有糖皮质激素治疗的病例中均升高，且与空泡化程度无特异性。对诊断为异常肝脏的牛血清和肝组织进行检测，以确定血清ADA与肝脏单核细胞浸润程度的关系。血清ADA升高，肝脏炎症细胞浸润增加。因此，奶牛血清ADA分析可能是肝脏炎症变化的预测指标。说明犬血清中ADA活性在保存条件下是不稳定的。犬淋巴瘤、白血病、肝肿瘤、脑膜瘤患者化疗前血清ADA升高。化疗后ADA水平有降低的趋势。其波动与肿瘤患者ALP或乳酸脱氢酶（LDH）波动具有不同的临床意义。我们认为狗的ADA分析可能为肿瘤行为提供一种预测指标。