STUDY ON CLINICAL EVALUATION OF SERUM AND URINE ENZYMES IN ORDER TO IMPROVE ACCURACYIN VETERINARYMEDICINE

用全自动生化分析仪对牛、犬肝脏和肿瘤患者血清碱性磷酸酶(ALP)和腺苷脱氨酶(ADA)进行了测定。我们建立了血清ALP同工酶的定量方法，结合白胚凝集素沉淀和左旋咪唑化学抑制骨ALP(BALP)和肝脏ALP(LALP)，分别对BALP和皮质类固醇诱导性ALP(CALP)进行了定量。由于CALP和BALP是已知的，因此LALP由公式ALP=BALP+LALP+CALP计算出来。本文讨论了血清ALP同工酶与糖皮质激素所致肝病程度的关系。将ALP、LALP和CALP的变化与肝实质空泡化的变化进行比较，发现CALP的升高与空泡化的严重程度直接相关，而LALP不同于糖皮质激素治疗后的LALP，LALP的升高与空泡化的程度无关。对诊断为肝脏异常的牛血清和肝组织进行检测，以确定血清ADA与肝脏单个核细胞浸润程度的关系。血清ADA升高，肝组织炎性细胞浸润增多。因此，奶牛血清ADA分析可能是肝脏炎症变化的预测指标。提示在保存条件下，犬血清中的ADA活性是不饱和的。犬淋巴瘤、白血球、肝肿瘤、脑膜瘤患者化疗前血清ADA升高。化疗后ADA水平呈下降趋势。其变化与肿瘤患者ALP或乳酸脱氢酶(LDH)的变化具有不同的临床意义。我们认为，犬的ADA分析可能为肿瘤行为提供一个预测指标。

Serum alkaline phosphates (ALP) and adenosine deaminase (ADA) were evaluated in the cases of liver and tumor diseases in cows and dogs by using an auto analyzer. We had established a quantification method for that ALP isoenzymes in the serum combined with white germ lectin precipitation and chemical inhibition of bone ALP (BALP) and liver ALP (LALP) isoenzymes with levamisole to quantify BALP and corticosteroid-induced ALP (CALP), respectively. Because CALP and BALP were known, LALP was calculated from the equation : ALP=BALP+LALP+CALP.We discussed with the relationship between serum ALP isoenzymes and the degree of glucocorticoid induced hepatopathy. The changes in ALP, LALP and CALP were compared to changes in vacuolization of the hepatic parenchyma CALP elevation was found to directly correlate with the severity of vacuolization unlike LALP that increased in all cases treated with glucocorticoids and was non-specific to the degree of vacuolization. Bovine serum and liver tissues which were diagnosed as being from abnormal livers were investigated to determine the relationship between the serum ADA and the degree of mononuclear cell infiltration in the liver. The serum ADA was elevated accompanied by an increase of inflammatory cell infiltration in the liver. Thus, serum ADA analysis of cows might be a predictive index of inflammatory change in the liver. It was suggested that the ADA activity in the canine serum is insatable in the condition of preservation. Serum ADA in cases of canine lymphoma, leukeamia, liver tumor and meningioma increased before chemotherapy. The ADA levels tended to decrease after chemotherapy. Its fluctuation has different clinical significance to that of ALP or lactate dehydrogenase (LDH) in the cases of tumor disorders. We suggest that ADA analysis in dogs may provide one predictive index for tumor behavior.