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Establishment of various measures against infectious diseases between domestic and wildlife animals, preventing the development of stock breeding in southern Africa

制定各种措施防治家畜和野生动物之间的传染病，阻碍南部非洲畜牧业的发展

基本信息

批准号：
15255021
负责人：
YASUDA Jun
金额：
$ 25.88万
依托单位：
IWATE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

项目摘要

1.TRANSMISSIBLE DISEASES BETWEEN DOMESTIC AND WILD ANIMALSSerological surveillance of BIV was conducted in traditional cattle in Zambia. BIV pol gene products showed 98.0-100% homology to the American strain. DNA sequences detected in springboks showed 44-72% homology to the Gammaherpesvirinae, indicating unrecognized novel virus. From nucleotide sequences of the VP2 hypervariable region of infectious bursal disease viruses in Tanzania, both African and European/Asian variants were demonstrated.2.DEVELOPMENT OF ANTI-TICK VACCINERapid amplification of the cDNA ends and primers designed based on a conserved seprin were used for the protease inhibitor from Haemaphysalis longicornis as an anti-tick vaccine. Vaccination of cattle with a combination of Rhipivcephalus appendiculutus seprine inhibitor conferred protective immunity against ticks. This is the first report on an anti-tick vaccine trial.3.DEVELOPMENT OF NEW DIAGNOSTIC TECHNOLOGYSpecies-specific loop-mediated isothermal amplification (LAMP) method is a novel nucleic acid method. We used this method for the diagnosis of equine piroplasmosis and bovine theileriosis and trypanosomosis. We tried a 2-step method using isothermal and chimeric primer-initiated amplification of nucleic acids to amplify the Salmonella invA and detecting the amplified product using ELISA from carcass samples in Zambia.

1.家畜与野生动物之间的传染病在赞比亚对传统牛进行了BIV血清学监测。BIV pol基因产物与美洲株同源性为98.0 ~ 100%。在跳羚中检测到的DNA序列与伽玛疱疹病毒科的同源性为44-72%，表明未识别的新型病毒。从坦桑尼亚传染性法氏囊病病毒VP2高变区核苷酸序列中，证实了非洲和欧洲/亚洲的变异。抗蜱疫苗的研制利用长角血蜱蛋白酶抑制剂cDNA末端的快速扩增和基于保守分离蛋白设计的引物作为抗蜱疫苗。给牛接种阑尾根头虫seprine抑制剂组合疫苗，可获得对蜱虫的保护性免疫。这是关于抗蜱疫苗试验的第一份报告。新诊断技术的发展种特异性环介导等温扩增（LAMP）方法是一种新型的核酸检测方法。我们将此方法应用于马体浆虫病、牛伊犁菌病和锥虫病的诊断。本研究采用等温和嵌合引物启动核酸扩增两步法对赞比亚产的invA沙门氏菌进行扩增，并用ELISA法对扩增产物进行检测。

项目成果

期刊论文数量（63）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Adenosine Deaminase Activity in Cats Infected with Feline Immunodeficiency Virus.

感染猫免疫缺陷病毒的猫的腺苷脱氨酶活性。

DOI：
发表时间：
2008
期刊：
Journal of Veterinary Medical Science 69
影响因子：
0
作者：
Careen HANKANGA;Saori KOBAYASHI;Yuichi YAMADA;Yutaka MOMOTA;Nobuyuki TOMIZAWA;Reeko SATO and Jun YASUDA
通讯作者：
Reeko SATO and Jun YASUDA

Isolation of Rhodococcus equi from the feces of indigenous animals an soil from the Lower Zambezi National Park and Lochinvar National Park Zambia

从赞比西河下游国家公园和赞比亚洛钦瓦尔国家公园的本土动物粪便和土壤中分离马红球菌