权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

TRANSMISSIBLE DISEASES BETWEEN DOMESTIC AND WILD ANIMALS IN AFRICA

非洲家养动物和野生动物之间的传染性疾病

基本信息

批准号：
11691165
负责人：
YASUDA Jun
金额：
$ 21.28万
依托单位：
IWATE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11691165/
关键词：
ZAMBIA CATTLE KAFUE LECHWE GENE ANALYSIS BOVINE HERPES VIRUS 1 LIVER FLUKES BOVINE IMMUNODEFICIENCY VIRUS BOVINE TUBERCULOSIS 野生動物ウシ免疫不全ウイルスBIV ウシヘルペスウイルスBHV-1 精子形成原虫病相互伝播性疾患重金属汚染雌雄鑑別ヘルペスウィルスタイレリア

项目摘要

The prevalence of the BHV 1 antigens in cattle was 23.28 % while the mean prevalence of the BHV 1 antibodies was 48.28 %. The prevalence of the Rabies in Zambia was 3-28.8 %, especially in dogs and cattle was 75 % and 13 %, respectively. As the nucleotide sequences of dogs showed 93-99 % homology to wild beasts in Zambia, it was concluded that wild beasts were infected Rabies from dogs. The liver flukes obtained from cattle and Kafue lechwe were identified as Fasciola gigantica with normal spermatogenesis ability. The flukes from cattle showed three different genotypes (A, B and C), but those from lechue did one genotype C. Since phylogenetical tree showed that the genotype C was genetically distinct from the genotypes A and B, the liver flukes of genotype C was considered to be spread to cattle from lechwe. The tuberculosis due to Mycobacterium bovis is present in lechwe showing pathological changes as seen in cattle. The DNA finger printing of the isolates from different localities e … More xhibited genetic similarity suggesting that the same strain is circulating in the lechwe population and probably in cattle causing bovine tuberculosis. Theileria was not found in lechwe from both blood smear samples and PCR analysis, while 7 % samples from cattle were recognized to have rRNA by PCR. Four species of Theileria were detected from cattle and buffalo. Rhodococcus equi isolated from zebra had no pathological marker protein and plasmid. It was suggested the infection circle between domestic horse. Antibodies to influenza A virus were detected 8 % samples of donkeys from Zambia by ELISA. ELISA-positive serum samples were subjected to HI test. Four positive samples inhibited hemagglutination of A/Aichi/2/68 but not of equine isolates. It is known that hemagglutination of avian and equine influenza viruses tend not to be inhibited by their anti-hemagglutinin antibodies. Seroprevalence of BlV in cattle was 12 %. BIV seropositive samples were found to have BIV provirus-DNA by nested PCR. The nucleotide sequences of Zambian BIV isolates showed highly homology to BIV-R29. Less

牛中BHV - 1抗原的感染率为23.28%，BHV - 1抗体的平均感染率为48.28%。赞比亚狂犬病的流行率为3- 28.8%，其中犬和牛的流行率分别为75%和13%。犬类与赞比亚野生动物的核苷酸序列同源性为93 ~ 99%，认为野生动物感染狂犬病来源于犬类。牛肝吸虫和卡富乳牛肝吸虫经鉴定为巨片吸虫，生精能力正常。牛肝吸虫有3种不同的基因型（A、B、C），而猪粪肝吸虫有1种基因型(C)。系统进化树显示，C基因型与A、B基因型有明显的遗传差异，可以认为C基因型的猪肝吸虫是从猪粪传播到牛的。由牛分枝杆菌引起的结核病存在于奶牛中，表现出与牛一样的病理变化。来自不同地区的分离株的DNA指纹图谱显示出遗传相似性，表明同一菌株在奶牛种群中传播，并且可能在引起牛结核病的牛中传播。乳牛血液涂片和PCR均未检出该菌，而牛血清中有7%的样本含有rRNA。从牛和水牛中检出4种伊氏菌。从斑马中分离得到的马红球菌无病理标记蛋白和质粒。提出了家马之间的感染圈。采用酶联免疫吸附法在8%的赞比亚驴样本中检测到甲型流感病毒抗体。elisa阳性血清标本进行HI试验。4个阳性样本对A/爱知/2/68的血凝作用有抑制作用，而对马分离株无抑制作用。众所周知，禽流感病毒和马流感病毒的血凝作用往往不受其抗血凝素抗体的抑制。牛BlV血清阳性率为12%。通过巢式PCR检测，发现乙肝病毒血清阳性样本含有乙肝病毒原病毒dna。赞比亚BIV分离株核苷酸序列与BIV- r29具有高度同源性。少