TRANSMISSIBLE DISEASES BETWEEN DOMESTIC AND WILD ANIMALS IN AFRICA
TRANSMISSIBLE DISEASES BETWEEN DOMESTIC AND WILD ANIMALS IN AFRICA
批准号:
11691165
负责人:
YASUDA Jun
金额:
$21.28万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (A)
财政年份:
1999
资助国家:
日本
项目状态:
已结题
起止时间:
1999 至 2001
中文摘要
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英文摘要
The prevalence of the BHV 1 antigens in cattle was 23.28 % while the mean prevalence of the BHV 1 antibodies was 48.28 %. The prevalence of the Rabies in Zambia was 3-28.8 %, especially in dogs and cattle was 75 % and 13 %, respectively. As the nucleotide sequences of dogs showed 93-99 % homology to wild beasts in Zambia, it was concluded that wild beasts were infected Rabies from dogs. The liver flukes obtained from cattle and Kafue lechwe were identified as Fasciola gigantica with normal spermatogenesis ability. The flukes from cattle showed three different genotypes (A, B and C), but those from lechue did one genotype C. Since phylogenetical tree showed that the genotype C was genetically distinct from the genotypes A and B, the liver flukes of genotype C was considered to be spread to cattle from lechwe. The tuberculosis due to Mycobacterium bovis is present in lechwe showing pathological changes as seen in cattle. The DNA finger printing of the isolates from different localities e … More xhibited genetic similarity suggesting that the same strain is circulating in the lechwe population and probably in cattle causing bovine tuberculosis. Theileria was not found in lechwe from both blood smear samples and PCR analysis, while 7 % samples from cattle were recognized to have rRNA by PCR. Four species of Theileria were detected from cattle and buffalo. Rhodococcus equi isolated from zebra had no pathological marker protein and plasmid. It was suggested the infection circle between domestic horse. Antibodies to influenza A virus were detected 8 % samples of donkeys from Zambia by ELISA. ELISA-positive serum samples were subjected to HI test. Four positive samples inhibited hemagglutination of A/Aichi/2/68 but not of equine isolates. It is known that hemagglutination of avian and equine influenza viruses tend not to be inhibited by their anti-hemagglutinin antibodies. Seroprevalence of BlV in cattle was 12 %. BIV seropositive samples were found to have BIV provirus-DNA by nested PCR. The nucleotide sequences of Zambian BIV isolates showed highly homology to BIV-R29. Less
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A.Tsuda: "cDNA cloning, characterization and vaccine effect analysis of Haemaphysalis longicomis tick saliva proteins"Vaccine. 19(30). 4287-4296 (2001)
A.Tsuda:“长血蜱唾液蛋白的cDNA克隆、表征及疫苗效果分析”疫苗。
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A. Mulenga, C. Sugimoto, M. Onuma: "Issues in tick vaccine development : identification and characterization of potencial candidate vaccine antigens. -Review-"Microbe & Infection. 2. 1353-1361 (2000)
A. Mulenga、C. Sugimoto、M. Onuma:“蜱疫苗开发中的问题:潜在候选疫苗抗原的识别和表征。-评论-”微生物
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A.Mulenga: "Characterization of two cDNAs encoding serine from the hard tick"Haemaphysalis longicomis. Insect Biochem. Molecular Biology. 31(8). 817-825 (2001)
A. Mulenga:“来自硬蜱”Haemaphysalis longicomis 的两种编码丝氨酸的 cDNA 的表征。
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通讯作者:
A. Tsuda, A. Mulenga, C. Sugimoto, M. Nakajima, K. Ohashi, M. Onuma: "cDNA cloning, characterization and vaccineeffect analysis of Haemaphysalis longicornis tick saliva proteins"Vaccine.. 19(30). 4287-4296 (2001)
A. Tsuda、A. Mulenga、C. Sugimoto、M. Nakajima、K. Ohashi、M. Onuma:“长角血蜱唾液蛋白的 cDNA 克隆、表征和疫苗效果分析”疫苗.. 19(30)。
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M. Syakarima, K. Choongo, Y. Nakazato, M. Onuma, C. Sugimoto, T. Tsubota, H. Fukushi, M. Yoshida, T. Itagaki, J. Yasuda: "An investigation of heavy metal exposure and risks to wildlife in the Kafue flats of Zambia"J. Vet. Med. Sci.. 63. 315-318 (2001)
M. Syakarima、K. Choongo、Y. Nakazato、M. Onuma、C. Sugimoto、T. Tsubota、H. Fukushi、M. Yoshida、T. Itagaki、J. Yasuda:“重金属暴露及其风险的调查
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