Mechanism of Asphyxial Congestion in Ligature Strangulation.

掐死豚鼠，观察窒息性充血时肺血管舒缩情况，采用荧光组织化学、PAP免疫细胞化学和放射自显影技术观察肺动脉和毛细血管内皮细胞对去甲肾上腺素摄取率的变化。放射线测定，窒息组大鼠肺动脉和毛细血管对去甲肾上腺素的摄取量显著高于对照组。在收缩的动脉中形成了许多肌内皮连接，PAP法显示这些连接区域有高浓度的去甲肾上腺素。这可能意味着肺充血增强了去甲肾上腺素从血浆通过内皮层向平滑肌细胞的转移。根据目前的实验，可以认为，肺血管收缩是由血浆去甲肾上腺素流入肺动脉和毛细血管的内皮细胞引起的。放射分析显示窒息肺组织的组胺浓度增加。免疫电镜观察组胺仅在窒息肺静脉内皮表面发生重反应。这些数据强烈提示肺静脉内皮细胞依赖性血管扩张是由内皮表面的h1受体介导的。采用放射自显像法和免疫细胞化学法观察了绞勒豚鼠脾、肾血管壁中肾上腺素、去甲肾上腺素和组胺的定位，并测定了绞勒处死的绞勒豚鼠脾等组织中^ 3h -肾上腺素和^ 3h -去甲肾上腺素含量。肾上腺素给药后，实验组和对照组脾中心动脉、脾小梁静脉、脾静脉窦和肾小叶间动脉内皮细胞中均可见银颗粒。脾、肾组织中^ 3h -肾上腺素含量明显高于对照组。从上述事实可以看出肾上腺素引起的脾中央动脉、脾小梁静脉和脾静脉窦的收缩。少

Guinea-pigs were killed by strangulation to investigate the pulmonary vasomotion during asphyxial congestion, and changes in rate of noradrenaline uptake by endothelial cells of the pulmonary arteries and capillaries were investigated with fluorescence histochemistry, PAP immunocytochemistry, and autoradiography. By radioassay, the amount of noradrenaline uptake by the pulmonary arteries and capillaries in the strangulation group was at a significantly higher level than that in control groups. Many myoendothelial junctions were formed in the constricted arteries, and the PAP method indicated high concentrations of noradrenaline in these junctional regions. This may mean that the transfer of noradrenaline from blood plasma to the smooth muscle cells through the endothelial layer was enhanced by the pulmonary congestion. Based on the present experiments, it can be considered that the pulmonary vasoconstriction in strangulation is induced by influx of the plasma noradrenaline into endothe … More lial cells of the pulmonary arteries and capillaries. The radioassay indicated an increase in histamine concentrations of the asphyxial pulmonary tissue. Heavy reactions of histamine exclusively on the endothelial surface of the asphyxial pulmonary veins were determined by the immunoelectron microscopy. These data strongly suggest that the endothelial cell-dependent vasodilatation of the pulmonary veins is mediated by H1-receptor on the endothelial surface. The authors observed the localization of adrenaline, noradrenaline and histamine in the walls of splenic and renal blood vessels of strangulated guinea-pigs by autoradiography and immunocytochemistry, and measured the ^3H-adrenaline and ^3H-noradrenaline contents of the spleen et al's tissues of the strangulated guinea-pigs sacrificed by ligature strangulation. In both the experimental and control groups, some silver grains were observed in the endothelial cells of splenic central arteries, splenic trabecular veins, splenic venous sinuses and renal interlobular arteries after ^3H-adrenaline administrations. By radioassay, the ^3H-adrenaline contents of splenic and renal tissues were more than the control groups. From the above-mentioned facts, adrenaline-induced constrictions of splenic central arteries, splenic trabecular veins and splenic venous sinuses were recognized. Less