Mechanism of Asphyxial Congestion in Ligature Strangulation.

结扎勒死中窒息充血的机制。

• 批准号: 63570280
• 负责人: KITA Toshiro
• 金额: $ 1.28万
• 依托单位: University of Occupational and Environmental Health, Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570280/
• 关键词: Asphyxia; Ligature strangulation; Pulmonary congestion; Adrenaline; Noradrenaline; Spleen anemia; Histamine; 窒息; 内臓うっ血; 静脈性うっ血; 肺動脈および肺毛細血管収縮; 肺静脈拡張; 脾臓収縮; カテコールアミン; ヒスタミン

Guinea-pigs were killed by strangulation to investigate the pulmonary vasomotion during asphyxial congestion, and changes in rate of noradrenaline uptake by endothelial cells of the pulmonary arteries and capillaries were investigated with fluorescence histochemistry, PAP immunocytochemistry, and autoradiography. By radioassay, the amount of noradrenaline uptake by the pulmonary arteries and capillaries in the strangulation group was at a significantly higher level than that in control groups. Many myoendothelial junctions were formed in the constricted arteries, and the PAP method indicated high concentrations of noradrenaline in these junctional regions. This may mean that the transfer of noradrenaline from blood plasma to the smooth muscle cells through the endothelial layer was enhanced by the pulmonary congestion. Based on the present experiments, it can be considered that the pulmonary vasoconstriction in strangulation is induced by influx of the plasma noradrenaline into endothe … More lial cells of the pulmonary arteries and capillaries. The radioassay indicated an increase in histamine concentrations of the asphyxial pulmonary tissue. Heavy reactions of histamine exclusively on the endothelial surface of the asphyxial pulmonary veins were determined by the immunoelectron microscopy. These data strongly suggest that the endothelial cell-dependent vasodilatation of the pulmonary veins is mediated by H1-receptor on the endothelial surface. The authors observed the localization of adrenaline, noradrenaline and histamine in the walls of splenic and renal blood vessels of strangulated guinea-pigs by autoradiography and immunocytochemistry, and measured the ^3H-adrenaline and ^3H-noradrenaline contents of the spleen et al's tissues of the strangulated guinea-pigs sacrificed by ligature strangulation. In both the experimental and control groups, some silver grains were observed in the endothelial cells of splenic central arteries, splenic trabecular veins, splenic venous sinuses and renal interlobular arteries after ^3H-adrenaline administrations. By radioassay, the ^3H-adrenaline contents of splenic and renal tissues were more than the control groups. From the above-mentioned facts, adrenaline-induced constrictions of splenic central arteries, splenic trabecular veins and splenic venous sinuses were recognized. Less

用荧光组织化学、PAP免疫细胞化学和放射自显影技术观察了豚鼠窒息性肺淤血时肺血管舒缩功能的变化。放射性分析显示，绞窄组肺动脉和毛细血管摄取去甲肾上腺素的量显著高于对照组。收缩的动脉中形成了许多肌内皮连接，PAP法显示这些连接区域中有高浓度的去甲肾上腺素。这可能意味着去甲肾上腺素从血浆通过内皮层向平滑肌细胞的转移被肺充血所增强。根据本实验结果，认为绞窄性肺血管收缩是由血浆去甲肾上腺素内流到内皮细胞引起的。 ...更多信息 肺动脉和毛细血管的平滑肌细胞。放射性分析表明窒息肺组织组胺浓度增加。用免疫电镜观察窒息肺静脉内皮细胞表面组胺的反应。这些数据有力地表明，内皮细胞依赖的肺静脉血管舒张是由内皮细胞表面的H1受体介导的。作者用放射自显影和免疫细胞化学方法观察了肾上腺素、去甲肾上腺素和组胺在绞窄豚鼠脾和肾血管壁的定位，并测定了绞窄豚鼠脾等组织中^3 H-肾上腺素和^3 H-去甲肾上腺素的含量。在实验组和对照组中，给予^3 H-肾上腺素后，在脾中央动脉、脾小梁静脉、脾静脉窦和肾小叶间动脉的内皮细胞中观察到一些银颗粒。放射性分析显示，脾、肾组织中^3H-肾上腺素含量均高于对照组。从上述事实，肾上腺素引起的脾中央动脉，脾小梁静脉和脾静脉窦的收缩被确认。少

项目成果

北 敏郎: "絞死における肺うっ血の成因(VIII)" 医学研究. 59. 83-89 (1989)

Toshiro Kita：“勒死时肺充血的原因（VIII）”医学研究 59. 83-89（1989）。

Toshiro KITA: "Mechanisms of the pulmonary congestion in strangulation(VIII)." Igaku Kenkyu. 59. 83-89 (1989)

Toshiro KITA：“勒死时肺充血的机制（VIII）。”

Toshiro KITA: "Mechanisms of the pulmonary congestion in strangulation(VI)." Igaku Kenkyu. 59. 52-56 (1989)

Toshiro KITA：“勒死时肺充血的机制（VI）。”

北敏郎: Zeitschrift fur Rechtsmedizin.

Toshiro Kita：Rechtsmedizin 的 Zeitschrift。

kita Toshiro: "Histamine effects on pulmonary blood vessels in strangulation" Z Rechtsmed.103. 85-91 (1989)

kita Toshiro：“组胺对勒死时肺血管的影响”Z Rechtsmed.103。