The Effect of Aging on Intestinal Absorption at the Level of Membrane Biology.

膜生物学水平上的衰老对肠道吸收的影响。

• 批准号: 63570331
• 负责人: KIKUCHI Kazuhiro
• 金额: $ 1.34万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570331/
• 关键词: Intestine; Digestion and Absorption; Aging; 消化,吸収; 刷子縁膜; グルコ-ス; カルシウムイオン; リン酸イオン; Na^+@H^+ antiporter; 吸収; ベジクル; 輸送担体

[Effect of Aging on Glucose Uptake at the Brush Border Membrane]The uptake of ^<14>C-d-Glucose was investigated using rapid infiltration technique. Under the sodium gradient, the time course of the d-glucose uptake showed classical overshoot and its peak of the uptake was located at the 30 seconds. The uptake of glucose at the equilibrium was 200pmol/mg protein. No overshoot phenomenon was noted under the potassium gradient. These data depicted that the vesicles which used this experiment had not any contamination of cell organelles or basal lateral membranes. In the aged group, 48 months old rats, didn't showed overshoot phenomenon under the sodium gradient. The glucose uptake at the 30 seconds under the sodium gradient of the aged rats depicted 480 pmol/mg protein which was almost half amount of the control rats. The aged rats showed higher glucose uptake under the potassium gradient condition compared to the control rats. These data suggested that the functional changing of dーglucose transport with age.

[老化对刷状缘膜处葡萄糖摄取的影响]<14>使用快速渗透技术研究β C-d-葡萄糖的摄取。在钠梯度下，葡萄糖摄取呈典型的过冲现象，摄取高峰位于30秒。平衡时葡萄糖的摄取量为200 pmol/mg蛋白。在钾梯度下未观察到过冲现象。这些数据表明，使用本实验的囊泡没有任何细胞器或基底侧膜的污染。老龄组48月龄大鼠在钠梯度下未出现超调现象。老年大鼠在钠梯度下30秒时的葡萄糖摄取量为480 pmol/mg蛋白质，几乎是对照大鼠的一半。老年大鼠在钾梯度条件下葡萄糖摄取量明显高于对照组。提示葡萄糖转运功能随增龄而发生变化。

项目成果

Kazuhiro Kikuchi: "Intestinal Maturation ; Characterization of Na^+/ H^+ Exchanger on Brush Border Membrane in rat small intestine." Japanese Journal of Gastroenterology. vol. 86. 510 (1989)

Kazuhiro Kikuchi：“肠道成熟；大鼠小肠刷状缘膜上 Na^ / H^ 交换器的表征。”

菊地 登志子: "小腸細胞ミトコンドリアにおける無機燐酸輸送機構とその発達について" 日本消化器病学会誌(臨時増刊号). 86. 1984 (1989)

Toshiko Kikuchi：“无机磷酸盐转运机制及其在小肠细胞线粒体中的发展”日本胃肠病学会杂志（特刊）86。1984（1989）。

菊地 登志子: "小腸細胞内ミトコンドリアにおけるカルシウム輸送機構とその発達" 日本消化器病学会誌(臨時増刊号). 85. 2070 (1988)

Toshiko Kikuchi：“小肠细胞线粒体中的钙转运机制及其发展”日本胃肠病学会杂志（特刊）85。2070（1988）。

Toshiko Kikuchi: "Intestinal Maturation ; Characterization of Mitochondrial Phosphate Transport in the Rat." Japanese Journal of Gastroenterology. vol. 86. 1984 (1989)

Toshiko Kikuchi：“肠道成熟；大鼠线粒体磷酸盐运输的特征。”

Kazuhiro Kikuchi: "Effect of Dietary Polyamines on Regulation of Intestinal Na^+/H^+ Antiporter." Japanese Journal of Gastroenterology. vol. 86. 1984 (1989)

Kazuhiro Kikuchi：“膳食多胺对肠道 Na^ /H^ 逆向转运蛋白调节的影响。”