The Effect of Aging on Intestinal Absorption at the Level of Membrane Biology.

[玻璃杯老化对刷子边界膜的影响]^<14>C-d-玻璃杯使用快速渗透技术进行了调查。在钠梯度下，D-葡萄糖起飞的时间课程显示了经典的超载，其起飞的高峰在30秒内被定位。在equilibrium中提取葡萄糖为200 pmol/毫克蛋白质。没有超射现象被注意到在植物梯度下。这些数据依赖于那些使用过的实验并没有对细胞有机体或基本的长期薄膜进行任何接触。在年龄组， 48个月的老老鼠，并没有显示出在钠梯度下的超射现象。葡萄糖在30秒内上升到年龄大鼠的钠梯度为480 pmol/mg，其中蛋白质的控制大鼠的一半数量。年龄较大的老鼠表现出较高的葡萄糖水平，并将其与控制老鼠相比。这些数据提出了D-glucose传输与年龄的功能性变化。

[Effect of Aging on Glucose Uptake at the Brush Border Membrane]The uptake of ^<14>C-d-Glucose was investigated using rapid infiltration technique. Under the sodium gradient, the time course of the d-glucose uptake showed classical overshoot and its peak of the uptake was located at the 30 seconds. The uptake of glucose at the equilibrium was 200pmol/mg protein. No overshoot phenomenon was noted under the potassium gradient. These data depicted that the vesicles which used this experiment had not any contamination of cell organelles or basal lateral membranes. In the aged group, 48 months old rats, didn't showed overshoot phenomenon under the sodium gradient. The glucose uptake at the 30 seconds under the sodium gradient of the aged rats depicted 480 pmol/mg protein which was almost half amount of the control rats. The aged rats showed higher glucose uptake under the potassium gradient condition compared to the control rats. These data suggested that the functional changing of dーglucose transport with age.