Establishment of reproductive technology by centri-fusion method for porcine oocytes

猪卵母细胞离心融合繁殖技术的建立

• 批准号: 22380153
• 负责人: KIKUCHI Kazuhiro
• 金额: $ 12.48万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380153/
• 关键词: 応用動物; 発生・分化; 発生工学; 細胞操作; 受精; 発生分化; 発生・分

The objective of the study is to establish reproductive biotechnologies without micromanipulation but by fusion of ooplasmic fragments obtained after centrifugations of porcine oocytes. The fragments were consisted of brownish and transparent ones; the former one contains a lot of mitochondria. The oocytes reconstructed from brownish fragments showed better fertilization ability. The oocytes showing low developmental ability were fused with the fragments, their fertilization and developmental abilities were improved, of which abilities were not different between two types of fragments. Furthermore, fusion of the fragments prepared from fertilized oocytes with the matured ones showed oocyte activation ability. These findings are now expected for further improvements of the technologies.

本研究的目的是建立不需要显微操作，但通过融合猪卵母细胞离心后获得的卵浆片段的生殖生物技术。结果表明，细胞碎片由棕黄色和透明两部分组成，棕黄色碎片中含有大量线粒体。棕色碎片重构的卵母细胞具有较好的受精能力。将发育能力低下的卵母细胞与碎片融合，其受精能力和发育能力得到提高，两种类型的碎片之间的受精能力和发育能力没有差异。此外，从受精卵制备的片段与成熟的融合显示卵母细胞激活能力。这些发现现在有望进一步改进这些技术。

项目成果

Xenografting of gonadal tissues into mice as a possible method for conservation and utilization of porcine genetic resources

将性腺组织异种移植到小鼠体内作为保护和利用猪遗传资源的一种可能方法

• DOI: 10.1111/j.1740-0929.2011.00919.x
• 发表时间: 2011
• 期刊: Anim Sci J
• 影响因子: 2
• 作者: Kikuchi K;Nakai M;Kashiwazaki N;Kaneko H
• 通讯作者: Kaneko H

New perspectives of in vitro gamete preservation: Xenografting of testicular tissues

体外配子保存的新视角：睾丸组织异种移植

• 发表时间: 2013
• 作者: Kikuchi K;Nakai M;Somfai T;Noguchi J;Tanihara F;Ito J;Kashiwazaki N;Kaneko H
• 通讯作者: Kaneko H

Evaluation of zona pellucida function for sperm penetration during in vitro fertilization in pigs.

• DOI: 10.1262/jrd.2013-021
• 发表时间: 2013
• 期刊: The Journal of reproduction and development
• 作者: Tanihara F;Nakai M;Kaneko H;Noguchi J;Otoi T;Kikuchi K
• 通讯作者: Kikuchi K

Recent progress on pig genetic resources : Cryopreservation of gametes and gonadal tissues

猪遗传资源的最新进展：配子和性腺组织的冷冻保存

• 发表时间: 2013
• 作者: Kikuchi K;Kashiwazaki N ほか
• 通讯作者: Kashiwazaki N ほか

Contribution of mitochondria in restoration of fertilization and developmental competence of oocytes with low cytoplasmic maturity

线粒体对低胞质成熟度卵母细胞受精和发育能力恢复的贡献

• 发表时间: 2012
• 作者: Viet Linh N;Kikuchi K
• 通讯作者: Kikuchi K