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Studies on Hypothalamic CRHmRNA

下丘脑CRHmRNA的研究

基本信息

批准号：
63570536
负责人：
HASHIMOTO Kozo
金额：
$ 1.28万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

Corticotropin Releasing hormone (CRH) and Arginine Vaso-Pressin (AVP) are the most important physiological ACTH releasing factors. Gene expression of CRHmRNA and AVPmRNA were investigated. Levels of AVPmRNA in the hypothalamic Supra-Optic Nucleus (SON) and the Para-Ventricular Nucleus (PVN) were determined by semiquantitative in situ hybridization histochemistry. However, we failed to detect CRHmRNA levels by in situ hybridization technique in spite of great efforts.In bilaterally adrenalectomized rats, plasma ACTH levels increased significantly, CRH levels in the Median Eminence (ME) decreased and AVP levels in the ME increased one week after surgery. AVPmRNA in the PVN and SON increased significantly.In the rats given hypertonic saline (2%) for 3 days, CRH levels in the PVN and ME did not differ from those in the control rats. Plasma corticosterone, and AVP increased significantly. AVP contents in the posterior pituitary decreased and AVPmRNA in the PVN and SON increased significantly.Water deprivation increased AVPmRNA in the PVN and SON. Plasma levels of ACTH, corticosterone, AVP and osmolality were significantly elevated. The concentration in the posterior pituitary, ME and the remaining hypothalamic tissue did not differ significantly.Food deprivation decreased AVPmRNA in the PVN and SON. Plasma levels of ACTH and corticosterone were greatly elevated, while plasma AVP and osmolality did not differ significantly. The results indicated that food deprivation stimulated the pituitaryadrenal axis and that a reduction in AVPmRNA levels in food deprived rats was caused by food deprivation per se.According to these observations, AVP may be at least partly responsible for ACTH elevation in adrenal insufficiency, hypertonic saline administration and water deprivation but not in food deprivation.

促肾上腺皮质激素释放激素（CRH）和精氨酸血管加压素（AVP）是最重要的生理性促肾上腺皮质激素释放因子。检测CRHmRNA和AVP mRNA的基因表达。用半定量原位杂交组织化学方法检测下丘脑视上核（SON）和室旁核（PVN）内AVP mRNA的表达。双侧肾上腺切除大鼠术后1周，血浆ACTH水平显著升高，正中隆起（Median Eminence，ME）CRH水平降低，ME AVP水平升高。高渗盐水（2%）灌胃3天后，室旁核和肠系膜上核内CRH含量与对照组无明显差异。血浆皮质酮、AVP显著升高。垂体后叶AVP含量降低，PVN和SON中AVPmRNA表达增加，而PVN和SON中AVPmRNA表达增加。血浆ACTH、皮质酮、AVP和渗透压浓度显著升高。垂体后叶、ME和下丘脑组织中的AVPmRNA含量无显著性差异，食物剥夺使下丘脑室旁核和视上核中的AVPmRNA含量降低。血浆ACTH和皮质酮水平显著升高，而血浆AVP和渗透压没有显着差异。结果表明，食物剥夺可刺激垂体-肾上腺轴，食物剥夺可引起垂体-肾上腺轴AVPmRNA水平的降低，这可能是由于食物剥夺本身引起的，AVP可能参与了肾上腺皮质功能不全、高渗盐水和水剥夺时ACTH升高的部分过程，而与食物剥夺无关。